Hysteresis-like behavior of oxygen association-dissociation equilibrium curves of sickle cells determined by a new method
| Autor: | Adams B, Beaudoin Ag, Bartnicki De, Hiroshi Mizukami |
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| Rok vydání: | 1977 |
| Předmět: |
Polarography
Chemistry Polymers Drop (liquid) Hemoglobin Sickle Analytical chemistry chemistry.chemical_element Erythrocytes Abnormal Partial pressure Anemia Sickle Cell Hydrogen-Ion Concentration Oxygen General Biochemistry Genetics and Molecular Biology Blood cell Kinetics Structure-Activity Relationship medicine.anatomical_structure Spectrophotometry Oxyhemoglobins Electrode medicine Humans Deoxygenation Whole blood |
| Zdroj: | Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.). 154(2) |
| ISSN: | 0037-9727 |
| Popis: | SummaryAn instrument to determine the oxygen association-dissociation equilibrium of whole blood has been developed. Only one drop of blood sample is required to trace automatically the oxygen-red blood cell equilibrium curve in the direction of deoxygenation and reoxygenation within a total of less than 10 min. The whole blood is suspended in the form of a thin liquid film reenforced by a micromesh. The partial pressure of oxygen is changed continuously with a gas-mixing pump while simultaneously being recorded by polarographic oxygen electrodes. The spectral change of red blood cells is recorded by a dual-beam spec-trophotometer.Using this instrument it is shown that the oxygen equilibrium curve of sickle cells obtained during deoxygenation is shifted to a higher oxygen affinity as compared to those obtained during reoxygenation. This hysteresis-like behavior of equilibrium curves is also reflected in the Hill constant, n, which is greater for deoxygenation than for reoxygenation. On the other hand, if ... |
| Databáze: | OpenAIRE |
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