Multi-mycotoxin stable isotope dilution LC–MS/MS method for Fusarium toxins in beer
| Autor: | Marina Gotthardt, Jan Schüler, Katharina Habler, Michael Rychlik |
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| Rok vydání: | 2017 |
| Předmět: |
Fusarium
Indicator Dilution Techniques Food Contamination 01 natural sciences Analytical Chemistry chemistry.chemical_compound 0404 agricultural biotechnology Isotopes Limit of Detection Tandem Mass Spectrometry Fusarium toxins Sample preparation Solid phase extraction Mycotoxin Zearalenone Chromatography High Pressure Liquid Chromatography biology Chemistry 010401 analytical chemistry Beer 04 agricultural and veterinary sciences General Medicine Mycotoxins biology.organism_classification 040401 food science Beauvericin 0104 chemical sciences Enniatin Chromatography Liquid Food Science |
| Zdroj: | Food Chemistry. 218:447-454 |
| ISSN: | 0308-8146 |
| DOI: | 10.1016/j.foodchem.2016.09.100 |
| Popis: | A stable isotope dilution LC-MS/MS multi-mycotoxin method was developed for 12 different Fusarium toxins including modified mycotoxins in beer (deoxynivalenol-3-glucoside, deoxynivalenol, 3-acetyldeoxynivalenol, 15-acetyl-deoxynivalenol, HT2-toxin, T2-toxin, enniatin B, B1, A1, A, beauvericin and zearalenone). As sample preparation and purification of beer a combined solid phase extraction for trichothecenes, enniatins, beauvericin and zearalenone was firstly developed. The validation of the new method gave satisfying results: intra-day and inter-day precision and recoveries were 1-5%, 2-8% and 72-117%, respectively. In total, 61 different organic and conventional beer samples from Germany and all over the world were analyzed by using the newly developed multi-mycotoxin method. In summary, deoxynivalenol, deoxynivalenol-3-glucoside, 3-acetyldeoxynivaleneol and enniatin B were quantified in rather low contents in the investigated beer samples. None of the other monitored Fusarium toxins like 15-acetyldeoxynivalenol, HT2- and T2-toxin, zearalenone, enniatin B1, A1, A or beauvericin were detectable. |
| Databáze: | OpenAIRE |
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