Mutagenesis via IS transposition in Deinococcus radiodurans

Autor: Pascale Servant, Samuel Mennecier, Suzanne Sommer, Adriana Bailone, Geneviève Coste
Přispěvatelé: Institut de génétique et microbiologie [Orsay] (IGM), Centre National de la Recherche Scientifique (CNRS)-Université Paris-Sud - Paris 11 (UP11), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2006
Předmět:
Transposable element
MESH: Thymidylate Synthase
MESH: Genome
Archaeal
DNA Repair
DNA repair
Mutant
MESH: Heat
MESH: Genome
Bacterial
Microbiology
Transposition (music)
03 medical and health sciences
MESH: Protein Structure
Tertiary
MESH: Thermotoga maritima
Bacterial Proteins
[SDV.BBM]Life Sciences [q-bio]/Biochemistry
Molecular Biology
MESH: Models
Genetic
Insertion sequence
MESH: Bacterial Physiology
MESH: Phylogeny
MESH: Flavins
Molecular Biology
MESH: Thymidine Monophosphate
MESH: Bacterial Proteins
030304 developmental biology
MESH: Mutagenesis
Genetics
MESH: DNA Repair
0303 health sciences
biology
030306 microbiology
Point mutation
Mutagenesis
MESH: DNA
Deinococcus radiodurans
biology.organism_classification
MESH: Catalysis
MESH: Deinococcus
MESH: Temperature
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
MESH: DNA Transposable Elements
MESH: Archaea
DNA Transposable Elements
Deinococcus
MESH: Pyrococcus
Genome
Bacterial
MESH: Models
Molecular
Zdroj: Molecular Microbiology
Molecular Microbiology, Wiley, 2006, 59, pp.317-25
Molecular Microbiology, Wiley, 2006, 59 (1), pp.317-25. ⟨10.1111/j.1365-2958.2005.04936.x⟩
ISSN: 0950-382X
1365-2958
DOI: 10.1111/j.1365-2958.2005.04936.x⟩
Popis: Analysis of the complete genome indicates that insertion sequences (ISs) are abundant in the radio-resistant bacterium Deinococcus radiodurans. By developing a forward mutagenesis assay to detect any inactivation events in D. radiodurans, we found that in the presence of an active mismatch repair system 75% of the mutations to trimethoprim-resistance (Tmp(R)) resulted from an IS insertion into the thyA coding region. Analysis of their distribution among the spontaneous Tmp(R) mutants indicated that five different ISs were transpositionally active. A type II Miniature Inverted-repeat Transposable Element (MITE), related to one of the deinococcal ISs, was also discovered as an insertion into thyA. Seven additional genomic copies of this MITE element were identified by BLASTN. Gamma-ray irradiation of D. radiodurans led to an increase of up to 10-fold in the frequency of Tmp(R) mutants. Analysis of the induced mutations in cells exposed to 10 kGy indicated that gamma-irradiation induced transposition of ISDra2 approximately 100-fold. A 50-fold induction of ISDra2 transposition was also observed in cells exposed to 600 J m(-2) UV-irradiation. Point mutations to rifampicin resistance (Rif(R)) were also induced by gamma-irradiation to reach a plateau at 2 kGy. The plateau value represented a 16-fold increase in the mutant frequency over the background. Although error-free repair strategies predominate in D. radiodurans, an upregulation of transposition, as well as induction of point mutations in cells recovering from DNA damage, provide a genetic variability that may have long-term evolutionary consequences on the fitness of this organism in its habitat.
Databáze: OpenAIRE
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