Development of monoclonal antibodies for detection of necrotizing hepatopancreatitis in penaeid shrimp
| Autor: | Deborah Bradley-Dunlop, Carlos R. Pantoja, Donald V. Lightner |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Penaeidae
medicine.drug_class Immunoblotting Hepatopancreas Aquatic Science medicine.disease_cause Monoclonal antibody Polymerase Chain Reaction law.invention Microbiology chemistry.chemical_compound Microscopy Electron Transmission Species Specificity law Gram-Negative Bacteria medicine Animals Digoxigenin In Situ Hybridization Ecology Evolution Behavior and Systematics Polymerase chain reaction biology Campylobacter Antibodies Monoclonal biology.organism_classification Immunohistochemistry humanities Vibrio Shrimp chemistry |
| Zdroj: | Diseases of Aquatic Organisms. 60:233-240 |
| ISSN: | 1616-1580 0177-5103 |
| DOI: | 10.3354/dao060233 |
| Popis: | Monoclonal antibodies (MAbs) were produced against necrotizing hepatopancreatitis bacteria (NHP-B) of penaeid shrimp. The MAbs tested in dot-immunoblot (D-IB) assays were capable of detecting the NHP-B in hepatopancreas samples collected from moribund juvenile Litopenaeus vannamei during an experimentally induced NHP-B infection. The MAbs were also screened by immunohistochemistry (IHC) using case submissions that were determined to be infected not only by histology, but also polymerase chain reaction (PCR) and in situ hybridization (ISH) assays using specific digoxigenin (DIG)-labeled probes on histological sections prepared from naturally infected shrimp. Two of the MAbs were chosen for development of detection methods for NHP. The MAbs were tested using IHC methods on Davidson's alcohol-formalin-acetic acid (AFA) fixed tissue sections and identified NHP-B infected cells and tissues in a pattern similar to that seen with DIG-labeled NHP-specific gene probes. None of the MAbs reacted with tissue from specific pathogen-free (SPF) shrimp or with shrimp tissues infected with a rickettsia-like bacteria, Vibrio sp., Campylobacter sp., and Spiroplasma sp. The MAbs were found to be negative against these other organisms, demonstrating that they are species specific and useful for rapid diagnostic detection of NHP-B. |
| Databáze: | OpenAIRE |
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