Biologic Roles of Gangliosides GM3 and GD3 in the Attachment of Human Melanoma Cells to Extracellular Matrix Proteins
| Autor: | Masahiko Muto, Kenneth O. Lloyd, Junji Nakano, Hiroo Yasui |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
collagen
Integrins Receptors Collagen Skin Neoplasms medicine.drug_class Clone (cell biology) Dermatology Biology Monoclonal antibody Cell Line Extracellular matrix Type IV collagen laminin fibronectin Laminin Gangliosides hemic and lymphatic diseases medicine Animals G(M3) Ganglioside Humans Melanoma neoplasms Molecular Biology Extracellular Matrix Proteins Antibodies Monoclonal cell adhesion Cell Biology General Medicine Molecular biology carbohydrates (lipids) Fibronectin Cell culture biology.protein lipids (amino acids peptides and proteins) Chromatography Thin Layer Rabbits Antibody Biotechnology |
| Zdroj: | Journal of Investigative Dermatology Symposium Proceedings. 4:173-176 |
| ISSN: | 1087-0024 |
| DOI: | 10.1038/sj.jidsp.5640204 |
| Popis: | The biologic functions of gangliosides G(M3) and G(D3) in the attachment of human melanoma cells to extracellular matrix proteins (type I and IV collagens, fibronectin, and laminin) were investigated by using the G(D3)-deficient mutant clone (SK-MEL-28-N1) and the parent cell line SK-MEL28. SK-MEL-28-N1 (N1) (high G(M3) expression: G(M3), 97.3%; G(D3), 0%) was selected by treating SK-MEL-28 (high G(D3) but low G(M3): G(M3), 6.5%, G(D3), 93.5%) with an anti-G(D3) monoclonal antibody (R24) and rabbit complement and subsequent subcloning of the surviving cells. The N1 clone showed significantly higher ability to adhere to type I and IV collagens and laminin than the parent clone SK-MEL-28. In the N1 clone, the expression of alpha2beta1 and alpha3beta1 integrin receptors was increased, whereas in SK-MEL-28, their expression was very low or undetectable. The treatment with monoclonal antibodies directed specifically to G(D3) expressed on SK-MEL-28 inhibited the cell attachment to type IV collagen (33% inhibition of control), fibronectin (59%), and laminin (71%). These findings suggest that gangliosides G(M3) (by influencing integrin receptor levels) and G(D3) (by interacting directly with matrix proteins) might play some functional roles in attachment to extracellular matrix proteins and thereby enhance the metastatic potency of melanoma cells. |
| Databáze: | OpenAIRE |
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