Development of a Yeast Bio-Assay to Screen Anti-Mitochondrial Drugs
| Autor: | Johan Kock, Chantel Swart, Desmond Ncango, Johan Kock Jr., Ingrid Munnik, Marleen Maartens, Carolina Pohl, Pieter van Wyk |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Aspirin
Antifungal Agents Indazoles Hypha Anti-Inflammatory Agents Non-Steroidal Drug Evaluation Preclinical Lonidamine Antineoplastic Agents Riboflavin Biology Eremothecium Yeast Mitochondria Oxygen chemistry.chemical_compound chemistry Biochemistry Drug Discovery medicine Bioassay Biological Assay Oxylipins Salicylic acid Benzoic acid medicine.drug |
| Zdroj: | Current Drug Discovery Technologies. 6:186-191 |
| ISSN: | 1570-1638 |
| DOI: | 10.2174/157016309789054960 |
| Popis: | Previous studies show that acetylsalicylic acid (aspirin) at low concentrations affects yeast sexual structure development in a similar fashion than oxygen depletion. This is ascribed to its anti-mitochondrial action. In this study, we report the same for other anti-inflammatory (i.e. ibuprofen, indomethacin, salicylic acid, benzoic acid) as well as anticancer (Lonidamine) drugs, also known for inhibiting mitochondrial activity in mammalian cells. This is shown by a unique yeast bio-assay, with the mitochondrion-dependent sexual structure, riboflavin production, and hyphal morphology of the yeast Eremothecium ashbyi serving as indicators. These drugs affect this yeast in a similar way as found under oxygen limitation conditions by inhibiting sexual structure development (most sensitive), riboflavin production, and yielding characteristically wrinkled and granular hyphae, presenting a unique "anoxic" morphological pattern for this yeast. Only drugs associated with anti-mitochondrial activity presented such a pattern. This bio-assay may find application in the screening for novel drugs from various sources with anti-mitochondrial actions. |
| Databáze: | OpenAIRE |
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