Microbial interactions in the mosquito gut determineSerratiacolonization and blood-feeding propensity
| Autor: | Grant L. Hughes, George Golovko, Andrew Routh, Emily A. Hornett, Eva Heinz, Maria Pimenova, Yuriy Fovanov, Yiyang Zhou, Vsevolod L. Popov, Christopher M. Roundy, Elena V. Kozlova, Miguel A. Saldaña, Charles E. Hart, Enyia R Anderson, Kamil Khanipov, Shivanand Hegde, Scott C. Weaver |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Serratia
030231 tropical medicine Mosquito Vectors Aedes aegypti Microbiology Article Microbial ecology 03 medical and health sciences 0302 clinical medicine Aedes Animals Microbiome Symbiosis Ecology Evolution Behavior and Systematics 030304 developmental biology 0303 health sciences biology fungi biology.organism_classification Bacterial host response Phenotype Enterobacteriaceae Culex quinquefasciatus Gastrointestinal Microbiome Culex Serratia marcescens Microbial Interactions Bacteria |
| Zdroj: | ISME JOURNAL The ISME Journal |
| Popis: | How microbe–microbe interactions dictate microbial complexity in the mosquito gut is unclear. Previously we found that, Serratia, a gut symbiont that alters vector competence and is being considered for vector control, poorly colonized Aedes aegypti yet was abundant in Culex quinquefasciatus reared under identical conditions. To investigate the incompatibility between Serratia and Ae. aegypti, we characterized two distinct strains of Serratia marcescens from Cx. quinquefasciatus and examined their ability to infect Ae. aegypti. Both Serratia strains poorly infected Ae. aegypti, but when microbiome homeostasis was disrupted, the prevalence and titers of Serratia were similar to the infection in its native host. Examination of multiple genetically diverse Ae. aegypti lines found microbial interference to S. marcescens was commonplace, however, one line of Ae. aegypti was susceptible to infection. Microbiome analysis of resistant and susceptible lines indicated an inverse correlation between Enterobacteriaceae bacteria and Serratia, and experimental co-infections in a gnotobiotic system recapitulated the interference phenotype. Furthermore, we observed an effect on host behavior; Serratia exposure to Ae. aegypti disrupted their feeding behavior, and this phenotype was also reliant on interactions with their native microbiota. Our work highlights the complexity of host–microbe interactions and provides evidence that microbial interactions influence mosquito behavior. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|