Effects of diet-induced hypercholesterolemia on amyloid accumulation in ovariectomized mice
| Autor: | G. Victor Rajamanickam, V. Thanigavelan, V Kaliyamurthi |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Male
Amyloid medicine.medical_specialty medicine.drug_class Ovariectomy Blotting Western Hypercholesterolemia Estrogen receptor Enzyme-Linked Immunosorbent Assay Peptide General Biochemistry Genetics and Molecular Biology Cholesterol Dietary Mice chemistry.chemical_compound Western blot Internal medicine medicine Animals chemistry.chemical_classification medicine.diagnostic_test Cholesterol Estrogen Receptor alpha Brain General Medicine Immunohistochemistry surgical procedures operative Endocrinology chemistry Estrogen Ovariectomized rat Electrophoresis Polyacrylamide Gel Female General Agricultural and Biological Sciences hormones hormone substitutes and hormone antagonists |
| Zdroj: | Journal of Biosciences. 37:1017-1027 |
| ISSN: | 0973-7138 0250-5991 |
| DOI: | 10.1007/s12038-012-9262-y |
| Popis: | A central hypothesis in the study of Alzheimer's disease (AD) is the accumulation and aggregation of beta-amyloid peptide (A beta). Recent epidemiological studies suggest that patients with elevated cholesterol and decreased estrogen levels are more susceptible to AD through A beta accumulation. To test the above hypothesis, we used ovariectomized with diet-induced hypercholesterolemia (OVX) and hypercholesterolemia (HCL) diet alone mouse models. HPLC analysis reveals the presence of beta amyloid in the OVX and HCL mice brain. Congo red staining analysis revealed the extent of amyloid deposition in OVX and hypercholesterolemia mice brain. Overall, A beta levels were higher in OVX mice than in HCL. Secondly, estrogen receptors alpha (ER alpha) were assessed by immunohistochemistry and this suggested that there was a decreased expression of ER alpha in OVX animals when compared to hypercholesterolemic animals. A beta was quantified by Western blot and ELISA analysis. Overall, A beta levels were higher in OVX mice than in HCL mice. Our experimental results suggested that OVX animals were more susceptible to AD with significant increase in A beta peptide. |
| Databáze: | OpenAIRE |
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