Characteristics of steroidogenesis-related factors in the musk gland of Chinese forest musk deer (Moschus berezovskii)
| Autor: | Fangxia Yang, Chao Zhu, Jian Ma, Jinmeng Yang, Feng Shu, Chuanying Pan, Wuzi Dong, Xiang Li, Xueli Zheng, Daqian Dong, Guofan Peng |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty 3-Hydroxysteroid Dehydrogenases Moschus berezovskii medicine.drug_class Endocrinology Diabetes and Metabolism medicine.medical_treatment Clinical Biochemistry Biochemistry Transcriptome Fatty Acids Monounsaturated 03 medical and health sciences 0302 clinical medicine Endocrinology Exocrine Glands stomatognathic system Internal medicine Testis medicine Animals Secretion Testosterone Cholesterol Side-Chain Cleavage Enzyme Molecular Biology Gene Phylogeny biology Cholesterol side-chain cleavage enzyme Deer Steroid 17-alpha-Hydroxylase Cell Biology biology.organism_classification Androgen Steroid hormone 030104 developmental biology CYP17A1 030220 oncology & carcinogenesis Androgens Molecular Medicine |
| Zdroj: | The Journal of steroid biochemistry and molecular biology. 212 |
| ISSN: | 1879-1220 |
| Popis: | Musk secreted by Chinese forest musk deer (FMD; Moschus berezovskii) is a highly valuable ingredient in the fields of perfumery and medicine, and the main factor affecting the production of musk is the androgen level of male FMD. To clarify whether the musk gland of FMD can synthesize androgen, we compared and analyzed the expression patterns of steroid hormone biosynthesis-related genes in the musk gland and testis of FMD by RNA-seq and RT-qPCR. We obtained 33,308 and 38,602 unigenes from the musk gland and testis, respectively, and 26,780 co-expressed unigenes. Analysis of co-expressed genes revealed that 12,647 genes were annotated to 11,484 Gene Ontology terms and 10,941 genes were annotated to 6120 pathways, including several pathways important in metabolic and synthetic activity. Next, 21 steroid hormone synthesis-related genes were screened from the transcriptome of the musk gland of 4-month-old FMD. The expression levels of three key genes of steroid hormone biosynthesis (CYP11A1, CYP17A1, and HSD3B) in the musk gland differed from their expression levels in the testis based on RT-qPCR. Furthermore, immunohistochemistry indicated that CYP11A1, CYP17A1, and HSD3B were localized in the glandular tubular columnar cells of the musk gland. These results suggested that the musk gland of male FMD has the potential to locally synthesize steroid hormone and thus plays a critically important role in musk secretion. |
| Databáze: | OpenAIRE |
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