An origin of DNA replication from Lactococcus lactis bacteriophage c2
| Autor: | R. W. F. Le Page, M. W. Lubbers, N. R. Waterfield, A. W. Jarvis, K. M. Polzin |
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| Předmět: |
DNA Replication
viruses Genetic Vectors Molecular Sequence Data Replication Origin Siphoviridae Applied Microbiology and Biotechnology Conserved sequence chemistry.chemical_compound Intergenic region Shuttle vector Sequence Homology Nucleic Acid Escherichia coli Conserved Sequence Genetics Ecology biology Base Sequence Lactococcus lactis Nucleic acid sequence DNA replication biochemical phenomena metabolism and nutrition biology.organism_classification Molecular biology chemistry DNA Viral DNA Food Science Biotechnology Research Article |
| Zdroj: | Scopus-Elsevier |
| Popis: | An origin of DNA relication was identified in the intergenic region between the early and late gene regions of prolate lactococcal phage c2. A DNA fragment containing this origin, designated ori, was shown to direct DNA replication in Lactococcus lactis but not in Escherichia coli. A comparison of ori with the corresponding regions of other prolate phages revealed strict conservation of the nucleotide sequence in one half of this intergenic region. This conserved region alone would not support DNA replication. No open reading frames were identified in the ori fragment, suggesting that host factors alone are sufficient to initiate DNA replication at ori. A novel class of lactococcal vectors and E. coli-L. lactis shuttle vectors based on ori have been constructed. |
| Databáze: | OpenAIRE |
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