Evaluation of the sequence method as a tool to assess spontaneous baroreflex in reptiles
| Autor: | Driele Tavares, Gabriella Campos Rocha, Samanta Aparecida Castro, Karina F. Orsolini, Gustavo M. Oda, Renato Filogonio, Augusto S. Abe, Cleo A. C. Leite |
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| Rok vydání: | 2019 |
| Předmět: |
Nitroprusside
0106 biological sciences 0301 basic medicine Heartbeat Physiology Vasodilator Agents Blood Pressure Baroreflex Biology 010603 evolutionary biology 01 natural sciences Phenylephrine 03 medical and health sciences Heart Rate Vasoactive Heart rate Genetics Animals Vasoconstrictor Agents Molecular Biology Ecology Evolution Behavior and Systematics Sequence (medicine) musculoskeletal neural and ocular physiology Crotalus Reproducibility of Results Lizards 030104 developmental biology Blood pressure Iguanas cardiovascular system Animal Science and Zoology Neuroscience circulatory and respiratory physiology |
| Zdroj: | Journal of Experimental Zoology Part A: Ecological and Integrative Physiology. 331:374-381 |
| ISSN: | 2471-5638 |
| Popis: | The sequence method is an alternative to the traditional pharmacological approach (i.e., the Oxford technique) used to calculate baroreflex gain (G) in mammals. Although the sequence method assesses baroreflex by measuring spontaneous events of blood pressure regulation, the pharmacological method relies on the injection of vasoactive drugs that impact the baroreflex mechanism itself. The sequence method might be relevant for dynamic measurement of baroreflex modulation but it was never validated for vertebrates with low heart rate. Hence, we tested the sequence method in three species of reptiles and compared the results with those provided by the classic pharmacological method. G was similar between both methods and values correlated when parameters for the sequence method were set at delay 0 or 1 (i.e., the baroreflex system responds immediately to blood pressure changes or after 1 heartbeat). Calculation of the baroreflex effectiveness index was adequate at a minimum of 300 cycles and a delay of 1 for the three species. Therefore, the sequence method has been validated to investigate baroreflex regulation in reptiles, enabling studies during dynamic alterations in homeostasis. |
| Databáze: | OpenAIRE |
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