A surface-modified sperm sorting device with long-term stability
| Autor: | Gary D. Smith, Jason M. Wu, Kimberly J. Belford, Joerg Lahann, Yaokuang Chung, Shuichi Takayama |
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| Rok vydání: | 2006 |
| Předmět: |
Male
Materials science Sperm sorting Surface Properties Microfluidics Cell Culture Techniques Biomedical Engineering Analytical chemistry Cell Separation macromolecular substances Methacrylate Specimen Handling Contact angle chemistry.chemical_compound Humans Fourier transform infrared spectroscopy Molecular Biology Cells Cultured Sperm Count technology industry and agriculture Substrate (chemistry) Equipment Design Microfluidic Analytical Techniques Flow Cytometry Spermatozoa Equipment Failure Analysis chemistry Chemical engineering Ethylene glycol Protein adsorption |
| Zdroj: | Biomedical Microdevices. 8:99-107 |
| ISSN: | 1572-8781 1387-2176 |
| DOI: | 10.1007/s10544-006-7705-7 |
| Popis: | Microfluidic devices fabricated from poly (dime- thylsiloxane) (PDMS) offer the ability to improve our biological and medical capabilities. Although PDMS offers a range of intriguing benefits for biomedical applications, the intrinsically hydrophobic nature of PDMS may impede with the tremendous potential of these devices. Here, we describe a PDMS-based sperm sorting device, which has been surface-modified via graft-co-polymerization of poly(ethylene glycol) methyl ether methacrylate to create a moderately hydrophilic and non-fouling surface. This process involves the exposure of PDMS to UV/ozone, which activates the PDMS surface to bond to the substrate and, at the same time, initiates the graft-co-polymerization from the PDMS surface. In this study, we confirmed long-term stability of surface-modified PDMS for up to 56 days based on Fourier transformation infrared spectroscopy (FTIR), contact angle measurements, and protein adsorption studies. Moreover, the applicability of our method to PDMS-based sperm sorting devices was demonstrated by successfully sorting human sperm. |
| Databáze: | OpenAIRE |
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