In silico evidence of de novo interactions between ribosomal and Epstein - Barr virus proteins
| Autor: | Shruti Prashant Talwar, Edmund Ui Hang Sim |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Herpesvirus 4
Human Protein-protein interactions In silico Ribosomal proteins Ribosome biogenesis Computational biology Biology Protein Structure Secondary Protein–protein interaction Viral Proteins Ribosomal protein hemic and lymphatic diseases otorhinolaryngologic diseases Humans Epstein-Barr virus Computer Simulation Protein Interaction Maps lcsh:QH573-671 Molecular Biology Gene Regulation of gene expression EBNA1 lcsh:Cytology Viral translation Cell Biology Ribosomal RNA Molecular Docking Simulation stomatognathic diseases Gene Ontology Computational prediction Protein Binding Research Article |
| Zdroj: | BMC Molecular and Cell Biology, Vol 20, Iss 1, Pp 1-12 (2019) BMC Molecular and Cell Biology |
| ISSN: | 2661-8850 |
| DOI: | 10.1186/s12860-019-0219-y |
| Popis: | Background Association of Epstein-Barr virus (EBV) encoded latent gene products with host ribosomal proteins (RPs) has not been fully explored, despite their involvement in the aetiology of several human cancers. To gain an insight into their plausible interactions, we employed a computational approach that encompasses structural alignment, gene ontology analysis, pathway analysis, and molecular docking. Results In this study, the alignment analysis based on structural similarity allows the prediction of 48 potential interactions between 27 human RPs and the EBV proteins EBNA1, LMP1, LMP2A, and LMP2B. Gene ontology analysis of the putative protein-protein interactions (PPIs) reveals their probable involvement in RNA binding, ribosome biogenesis, metabolic and biosynthetic processes, and gene regulation. Pathway analysis shows their possible participation in viral infection strategies (viral translation), as well as oncogenesis (Wnt and EGFR signalling pathways). Finally, our molecular docking assay predicts the functional interactions of EBNA1 with four RPs individually: EBNA1-eS10, EBNA1-eS25, EBNA1-uL10 and EBNA1-uL11. Conclusion These interactions have never been revealed previously via either experimental or in silico approach. We envisage that the calculated interactions between the ribosomal and EBV proteins herein would provide a hypothetical model for future experimental studies on the functional relationship between ribosomal proteins and EBV infection. Electronic supplementary material The online version of this article (10.1186/s12860-019-0219-y) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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