New Immunochromatographic Method for Rapid Detection of Rotaviruses in Stool Samples Compared with Standard Enzyme Immunoassay and Latex Agglutination Techniques
| Autor: | Javier Colomina, Alicia Sánchez-Fauquier, Enriqueta Roman, D. Martín-Rodrigo, I. Wilhelmi |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Male
Rotavirus Microbiology (medical) medicine.disease_cause Sensitivity and Specificity Rapid detection Rotavirus Infections Microbiology Immunoenzyme Techniques Feces Predictive Value of Tests medicine Humans Chromatography medicine.diagnostic_test Diagnostic Tests Routine Reverse Transcriptase Polymerase Chain Reaction business.industry Infant General Medicine Acute gastroenteritis Predictive value Latex fixation test Infectious Diseases Child Preschool Immunoassay Predictive value of tests Immunoenzyme techniques Female business Latex Fixation Tests |
| Zdroj: | European Journal of Clinical Microbiology and Infectious Diseases. 20:741-743 |
| ISSN: | 1435-4373 0934-9723 |
| DOI: | 10.1007/s100960100575 |
| Popis: | Three different commercial immunologic tests for rapid detection of group A rotavirus (an immunochromatographic method, latex agglutination, and enzyme immunoassay) were used to evaluate 228 faecal specimens obtained from Spanish children with acute gastroenteritis. After resolution of 30 (13.2%) discordant results by reverse transcription-polymerase chain reaction for rotavirus, the statistical values of the enzyme immunoassay, latex agglutination, and immunochromatographic method were respectively 96%, 68%, and 99% for sensitivity; 99%, 99%, and 96% for specificity; 98%, 96%, and 92% for positive predictive value; and 98%, 88%, and 99% for negative predictive value. The immunochromatographic technique showed high sensitivity and specificity and was rapid and easy to perform in the routine clinical laboratory. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink