Leukocytes recruited by tumor-derived HMGB1 sustain peritoneal carcinomatosis
| Autor: | Cottone, Lucia, Capobianco, Annalisa, Gualteroni, Chiara, Monno, Antonella, Raccagni, Isabella, Valtorta, Silvia, Canu, Tamara, Di Tomaso, Tiziano, Lombardo, Angelo, Esposito, Antonio, Moresco, Rosa Maria, Del Maschio, Alessandro, Naldini, Luigi, Rovere-Querini, Patrizia, Bianchi, Marco E., Manfredi, Angelo A. |
|---|---|
| Přispěvatelé: | Cottone, L, Capobianco, A, Gualteroni, C, Monno, A, Raccagni, I, Valtorta, S, Canu, T, Tomaso, T, Lombardo, A, Esposito, A, Moresco, R, Maschio, A, Naldini, L, Rovere Querini, P, Bianchi, M, Manfredi, A, De Tomaso, T, Lombardo, ANGELO LEONE, Esposito, Antonio, Moresco, Rm, DEL MASCHIO, Alessandro, Naldini, Luigi, ROVERE QUERINI, Patrizia, Bianchi, MARCO EMILIO, Manfredi, ANGELO ANDREA M. A. |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
green fluorescent protein Pathology medicine.medical_specialty Murine colon adenocarcinoma cell line Phagocyte Macrophage Immunology Damage-associated molecular pattern chemical and pharmacologic phenomena High Mobility Box 1 macrophage HMGB1 GFP BoxA damage-associated molecular pattern Small hairpin RNA 03 medical and health sciences 0302 clinical medicine vascularization In vivo Leukocytes medicine Extracellular DAMP short hairpin RNA Immunology and Allergy MC-38 murine colon adenocarcinoma cell line Original Research Gene knockdown peritoneal carcinomatosi biology business.industry Macrophages Tumor-Derived Leukocyte 030104 developmental biology medicine.anatomical_structure Alarmin Oncology 030220 oncology & carcinogenesis biology.protein ShRNA business leukocyte Peritoneal carcinomatosis |
| Zdroj: | ONCOIMMUNOLOGY 5 (2016): e1122860. doi:10.1080/2162402X.2015.1122860 info:cnr-pdr/source/autori:Cottone, Lucia; Cottone, Lucia; Capobianco, Annalisa; Gualteroni, Chiara; Monno, Antonella; Raccagni, Isabella; Raccagni, Isabella; Valtorta, Silvia; Valtorta, Silvia; Canu, Tamara; Di Tomaso, Tiziano; Lombardo, Angelo; Lombardo, Angelo; Esposito, Antonio; Esposito, Antonio; Moresco, Rosa Maria; Moresco, Rosa Maria; Del Maschio, Alessandro; Del Maschio, Alessandro; Naldini, Luigi; Naldini, Luigi; Rovere-Querini, Patrizia; Rovere-Querini, Patrizia; Bianchi, Marco E.; Bianchi, Marco E.; Manfredi, Angelo A.; Manfredi, Angelo A./titolo:Leukocytes recruited by tumor-derived HMGB1 sustain peritoneal carcinomatosis/doi:10.1080%2F2162402X.2015.1122860/rivista:ONCOIMMUNOLOGY/anno:2016/pagina_da:e1122860/pagina_a:/intervallo_pagine:e1122860/volume:5 |
| DOI: | 10.1080/2162402X.2015.1122860 |
| Popis: | The factors that determine whether disseminated transformed cells in vivo yield neoplastic lesions have only been partially identified. We established an ad hoc model of peritoneal carcinomatosis by injecting colon carcinoma cells in mice. Tumor cells recruit inflammatory leukocytes, mostly macrophages, and generate neoplastic peritoneal lesions. Phagocyte depletion via clodronate treatment reduces neoplastic growth. Colon carcinoma cells release a prototypic damage-associated molecular pattern (DAMP)/alarmin, High Mobility Group Box1 (HMGB1), which attracts leukocytes. Exogenous HMGB1 accelerates leukocyte recruitment, macrophage infiltration, tumor growth and vascularization. Lentiviral-based HMGB1 knockdown or pharmacological interference with its extracellular impair macrophage recruitment and tumor growth. Our findings provide a preclinical proof of principle that strategies based on preventing HMGB1-driven recruitment of leukocytes could be used for treating peritoneal carcinomatosis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|