The Radioprotective Effect of Procaine and Procaine-Derived Product Gerovital H3 in Lymphocytes from Young and Aged Individuals

Autor: Gudrun von Scheven, Yves Artur, Claudia Borsa, Maria Moreno-Villanueva, Philippe Faure, Daniela Gradinaru, Lucie Oziol, Cristina Ionescu, Anca Ungurianu, Alexander Bürkle, Denisa Margina
Přispěvatelé: University of Medicine and Pharmacy 'Carol Davila' Bucharest (UMPCD), National Institute of Geriatrics and Gerontology 'Ana Aslan', University of Konstanz, Ecol Systemat & Evolut, UMR CNRS 8079, Centre National de la Recherche Scientifique (CNRS), Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Bihl, Ji C.
Rok vydání: 2020
Předmět:
Zdroj: Oxidative Medicine and Cellular Longevity
Oxidative Medicine and Cellular Longevity, Hindawi, 2020, 2020, pp.3580934. ⟨10.1155/2020/3580934⟩
Oxidative Medicine and Cellular Longevity, Vol 2020 (2020)
ISSN: 1942-0994
1942-0900
Popis: International audience; Ionizing radiation induces genomic instability in living organisms, and several studies reported an ageing-dependent radiosensitivity. Chemical compounds, such as scavengers, radioprotectors, and modifiers, contribute to reducing the radiation-associated toxicity. These compounds are often antioxidants, and therefore, in order to be effective, they must be present before or during exposure to radiation. However, not all antioxidants provide radioprotection. In this study, we investigated the effects of procaine and of a procaine-based product Gerovital H3 (GH3) on the formation of endogenous and X-ray-induced DNA strand breaks in peripheral blood mononuclear cells (PBMCs) isolated from young and elderly individuals. Interestingly, GH3 showed the strongest radioprotective effects in PBMCs from young subjects, while procaine reduced the endogenous amount of DNA strand breaks more pronounced in aged individuals. Both procaine and GH3 inhibited lipid peroxidation, but procaine was more effective in inhibiting mitochondria free radicals' generation, while GH3 showed a higher antioxidant action on macrophage-induced low-density lipoprotein oxidation. Our findings provide new insights into the mechanisms underlying the distinct effects of procaine and GH3 on DNA damage.
Databáze: OpenAIRE
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