Enhancer of zeste homolog 2 expression is associated with tumor cell proliferation and metastasis in gastric cancer
Autor: | Young Yiul Lee, Jin Sun Yoon, Young Soo Song, Kang Won Song, Jung Hye Choi |
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Rok vydání: | 2010 |
Předmět: |
Adult
Male Microbiology (medical) Cell macromolecular substances Pathology and Forensic Medicine Metastasis Cyclin D1 Stomach Neoplasms Cell Line Tumor medicine Humans Immunology and Allergy Enhancer of Zeste Homolog 2 Protein Neoplasm Metastasis Stomach cancer Aged Cell Proliferation Aged 80 and over biology Cell growth Polycomb Repressive Complex 2 Cancer General Medicine Middle Aged Cell cycle medicine.disease DNA-Binding Proteins Ki-67 Antigen medicine.anatomical_structure Ki-67 Cancer research biology.protein Female Transcription Factors |
Zdroj: | APMIS. 118:196-202 |
ISSN: | 1600-0463 0903-4641 |
Popis: | The enhancer of zeste homolog 2 (EZH2), a member of the polycomb group of proteins, plays an important role in cell proliferation and cell cycle regulation. EZH2 is overexpressed in aggressive forms of prostate, breast, bladder, and endometrial cancers. However, the role of EZH2 expression in gastric cancer has not been fully determined. This study was conducted to investigate the correlation between EZH2 and cell cycle-related molecules, and the clinical value of EZH2 expression in gastric cancer. We analyzed EZH2 expression using Western blotting in AGS, MKN-28, SNU-16, SNU-484, SNU-601, and SNU-638 gastric cancer cell lines. After transfection of EZH2 siRNA into MKN-28 cells, the change in cell cycle-related molecules was assessed by Western blot analysis. Expression of EZH2, Ki-67, and p53 was determined by immunohistochemical staining of tissue microarrays from specimens of 137 cases of resected gastric cancer. We found high expressions of EZH2 in all of the tested gastric cancer cell lines. RNA interference of EZH2 induced upregulation of p53 and HDAC1 and downregulation of cyclin D1 and cyclin E. High EZH2 expression was observed in 60.6% of gastric cancers and in 6.7% of non-neoplastic gastric tissues (p < 0.01); 40.1% were positive for p53 in gastric cancers. High EZH2 expression was correlated with Ki-67 and p53 expressions and was significantly associated with distant metastases and non-signet ring cells. Our results suggest that high EZH2 expression is associated with tumor cell proliferation and metastasis in gastric cancer. |
Databáze: | OpenAIRE |
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