Lifetime Ultraviolet Radiation Exposure and DNA Methylation in Blood Leukocytes: The Norwegian Women and Cancer Study

Autor: Therese Haugdahl Nøst, Arnoldo Frigessi, Torkjel M. Sandanger, Christian M. Page, Magne Thoresen, Reza Ghiasvand, Marit B. Veierød, Vera Djordjilović
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Adult
0301 basic medicine
Oncology
medicine.medical_specialty
Skin Neoplasms
Ultraviolet Rays
Settore SECS-P/05 - Econometria
lcsh:Medicine
Predictive markers
Article
03 medical and health sciences
0302 clinical medicine
visual_art.visual_artist
Sunbathing
Internal medicine
Leukocytes
Humans
Medicine
lcsh:Science
Aged
DNA methylation
Multidisciplinary
integumentary system
VDP::Medical disciplines: 700::Clinical medical disciplines: 750::Oncology: 762
Norway
business.industry
Melanoma
fungi
lcsh:R
Cancer
dNaM
Environmental Exposure
Environmental exposure
Methylation
Middle Aged
medicine.disease
3. Good health
VDP::Medisinske Fag: 700::Klinisk medisinske fag: 750::Onkologi: 762
030104 developmental biology
CpG site
030220 oncology & carcinogenesis
visual_art
CpG Islands
Female
lcsh:Q
business
Zdroj: Scientific Reports, Vol 10, Iss 1, Pp 1-8 (2020)
Scientific Reports
ISSN: 2045-2322
DOI: 10.1038/s41598-020-61430-3
Popis: Background Ultraviolet radiation (UVR) exposure is a leading cause of skin cancers and an ubiquitous environmental exposure. However, the molecular mechanisms relating UVR exposure to melanoma is not fully understood. We aimed to investigate if lifetime UVR exposure influences DNA methylation, and if individual CpG sites could be robustly associated with UVR exposures.Methods We assessed DNA methylation in whole blood in three data sets (N = 183, 191, and 125) from the Norwegian Women and Cancer cohort, using Illumina methylation platforms (450k & EPIC). We studied genome-wide DNA methylation, targeted analyses of CpG sites indicated in the literature, global methylation (average over all CpGs and imputation of LINE-1 specific CpGs), and accelerated aging. Lifetime history of UVR exposure (residential ambient UVR, sunburns, sunbathing vacations and indoor tanning) was collected by questionnaires. Cumulative UVR exposure was calculated by adding sunbathing vacations and indoor tanning. We used one data set for discovery and the other two for replication. Results One CpG site showed a genome-wide significant association between cumulative UVR exposure and DNA methylation (cg01884057) (pnominal=3.96e-08), but was not replicated in any of the two replication sets (pnominal≥0.42). Four CpG sites (cg05860019, cg00033666, cg18984282, cg25792367) showed suggestive associations with the other UVR exposures. Conclusion We performed extensive analyses of the association between long-term UVR exposure and DNA methylation in lymphocytes. There was no indication of a robust effect of past UVR exposure on DNA methylation, and our results do not suggest mediation of UVR effects on melanoma risk by DNA methylation.
Databáze: OpenAIRE
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