Reaction-diffusion hydrogels from urease enzyme particles for patterned coatings
| Autor: | Annette F. Taylor, Anthony Q. Mai, John A. Pojman, Tamás Bánsági |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Materials science
Urease Diffusion macromolecular substances 02 engineering and technology 010402 general chemistry 01 natural sciences Biochemistry Viscosity Materials Chemistry Environmental Chemistry QD1-999 biology Continuous reactor technology industry and agriculture Biofilm General Chemistry 021001 nanoscience & nanotechnology 0104 chemical sciences Chemistry Polymerization Chemical engineering Self-healing hydrogels biology.protein Particle 0210 nano-technology |
| Zdroj: | Communications Chemistry, Vol 4, Iss 1, Pp 1-7 (2021) |
| ISSN: | 2399-3669 |
| Popis: | The reaction and diffusion of small molecules is used to initiate the formation of protective polymeric layers, or biofilms, that attach cells to surfaces. Here, inspired by biofilm formation, we present a general method for the growth of hydrogels from urease enzyme-particles by combining production of ammonia with a pH-regulated polymerization reaction in solution. We show through experiments and simulations how the propagating basic front and thiol-acrylate polymerization were continuously maintained by the localized urease reaction in the presence of urea, resulting in hydrogel layers around the enzyme particles at surfaces, interfaces or in motion. The hydrogels adhere the enzyme-particles to surfaces and have a tunable growth rate of the order of 10 µm min−1 that depends on the size and spatial distribution of particles. This approach can be exploited to create enzyme-hydrogels or chemically patterned coatings for applications in biocatalytic flow reactors. Enzyme reaction networks can control the spatial and temporal formation of hydrogels. Here, inspired by biofilm formation, urease-containing particles create a propagating pH gradient which induces thiol-acrylate polymerization, with properties tuned by solution viscosity, particle dimensions, and spatial configuration. |
| Databáze: | OpenAIRE |
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