5(6)-Carboxyfluorescein Revisited: New Protecting Group, Separation of Isomers, and their Spectral Properties on Oligonucleotides
| Autor: | Vladimir A. Korshun, Dmitry A. Tsybulsky, Irina A. Stepanova, S. L. Bondarev, Alexey V. Ustinov, Sergey V. Gontarev, Vadim V. Shmanai, Maksim V. Kvach |
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| Rok vydání: | 2007 |
| Předmět: |
Pharmacology
Chromatography Cyclohexanecarboxylic Acids Oligonucleotide Organic Chemistry Oligonucleotides Biomedical Engineering Pharmaceutical Science Bioengineering Pentafluorophenyl esters Oligonucleotide synthesis Fluoresceins Mass spectrometry Fluorescence chemistry.chemical_compound Organophosphorus Compounds Spectrometry Fluorescence Column chromatography Isomerism chemistry Organic chemistry 6-Carboxyfluorescein Pentanoic Acids Protecting group Biotechnology |
| Zdroj: | Bioconjugate Chemistry. 18:1691-1696 |
| ISSN: | 1520-4812 1043-1802 |
| Popis: | Pentafluorophenyl esters of 5- and 6-carboxyfluorescein-3',6'-O-dipivalate can be easily separated in multigram quantities by column chromatography. The individual isomers were converted into stable phosphoramidites suitable for oligonucleotide synthesis. The use of the cyclohexylcarbonyl (Chc) protecting group instead of pivaloyl (Piv) facilitates the separation of isomers. The fluorescence spectra of 5- and 6-carboxyfluoresceins on oligonucleotides were compared. |
| Databáze: | OpenAIRE |
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