Sensitivity enhancement by exchange mediated magnetization transfer of the xenon biosensor signal
| Autor: | Lana Chavez, Sandra Garcia, Thomas J. Lowery, David E. Wemmer, Alexander Pines, Songi Han |
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| Rok vydání: | 2007 |
| Předmět: |
Remote detection
Nuclear and High Energy Physics Magnetic Resonance Spectroscopy Xenon Physics::Instrumentation and Detectors Physics::Medical Physics Biophysics Analytical chemistry chemistry.chemical_element Biosensing Techniques Sensitivity and Specificity Biochemistry Signal Molecular physics Magnetics Magnetization symbols.namesake Physics::Atomic and Molecular Clusters Magnetization transfer Microchemistry Reproducibility of Results Condensed Matter Physics chemistry Fourier analysis symbols Sensitivity (electronics) Biosensor Algorithms |
| Zdroj: | Journal of Magnetic Resonance. 184:72-77 |
| ISSN: | 1090-7807 |
| Popis: | Hyperpolarized xenon associated with ligand derivatized cryptophane-A cages has been developed as a NMR based biosensor. To optimize the detection sensitivity we describe use of xenon exchange between the caged and bulk dissolved xenon as an effective signal amplifier. This approach, somewhat analogous to 'remote detection' described recently, uses the chemical exchange to repeatedly transfer spectroscopic information from caged to bulk xenon, effectively integrating the caged signal. After an optimized integration period, the signal is read out by observation of the bulk magnetization. The spectrum of the caged xenon is reconstructed through use of a variable evolution period before transfer and Fourier analysis of the bulk signal as a function of the evolution time. |
| Databáze: | OpenAIRE |
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