CaLecRK-S.5, a pepper L-type lectin receptor kinase gene, confers broad-spectrum resistance by activating priming
| Autor: | Kyung Hee Paek, Young-Jin Kim, Joo Yong Woo, Kwang Ju Jeong |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Hypersensitive response Pepper mild mottle virus Physiology L-type lectin receptor kinase Plant Science Plant disease resistance Genes Plant 01 natural sciences Microbiology 03 medical and health sciences Gene Expression Regulation Plant Lectins Gene expression mental disorders Tobacco mosaic virus Gene silencing Gene Silencing priming Phylogeny Disease Resistance Plant Proteins Broad-spectrum resistance biology tobamovirus fungi food and beverages RNA sequencing biochemical phenomena metabolism and nutrition biology.organism_classification Virology Xanthomonas campestris CaLecRK-S.5 Enzyme Activation Tobacco Mosaic Virus 030104 developmental biology Capsicum Protein Kinases Sequence Alignment Systemic acquired resistance 010606 plant biology & botany Research Paper SAR |
| Zdroj: | Journal of Experimental Botany |
| ISSN: | 1460-2431 0022-0957 |
| Popis: | Highlight CaLecRK-S.5, a positive regulator of priming, plays a critical role in plant immunity to broad-spectrum pathogens. In Arabidopsis, several L-type lectin receptor kinases (LecRKs) have been identified as putative immune receptors. However, to date, there have been few analyses of LecRKs in crop plants. Virus-induced gene silencing of CaLecRK-S.5 verified the role of CaLecRK-S.5 in broad-spectrum resistance. Compared with control plants, CaLecRK-S.5-silenced plants showed reduced hypersensitive response, reactive oxygen species burst, secondary metabolite production, mitogen-activated protein kinase activation, and defense-related gene expression in response to Tobacco mosaic virus pathotype P0 (TMV-P0) infection. Suppression of CaLecRK-S.5 expression significantly enhanced the susceptibility to Pepper mild mottle virus pathotype P1,2,3, Xanthomonas campestris pv. vesicatoria, Phytophthora capsici, as well as TMV-P0. Additionally, β-aminobutyric acid treatment and a systemic acquired resistance assay revealed that CaLecRK-S.5 is involved in priming of plant immunity. Pre-treatment with β-aminobutyric acid before viral infection restored the reduced disease resistance phenotypes shown in CaLecRK-S.5-silenced plants. Systemic acquired resistance was also abolished in CaLecRK-S.5-silenced plants. Finally, RNA sequencing analysis indicated that CaLecRK-S.5 positively regulates plant immunity at the transcriptional level. Altogether, these results suggest that CaLecRK-S.5-mediated broad-spectrum resistance is associated with the regulation of priming. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|