Comparison of the biological properties of purified natural and recombinant human interleukin-2
| Autor: | Hiroko Tada, Koichi Kato, Masaru Koyama, Shuji Hinuma, K Naruo, Kyozo Tsukamoto, Osamu Shiho |
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| Rok vydání: | 1985 |
| Předmět: |
Time Factors
DNA Recombinant Biophysics Mice Nude Spleen Biology Biochemistry Cell Line law.invention Mice Nude mouse law medicine Animals Humans Molecular Biology Chromatography High Pressure Liquid Mice Inbred BALB C Lymphokine-activated killer cell Cell Biology biology.organism_classification Natural killer T cell Molecular biology In vitro Killer Cells Natural medicine.anatomical_structure Cell culture Recombinant DNA biology.protein Interleukin-2 Antibody Cell Division Thymidine |
| Zdroj: | Biochemical and Biophysical Research Communications. 128:257-264 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/0006-291x(85)91672-9 |
| Popis: | We compared the biological properties of the purified recombinant human IL-2 derived from E. coli with those of purified natural IL-2. Both had almost the same specific in vitro activities on a weight basis to support long-term proliferation of IL-2 dependent human peripheral blood lymphocytes, a mouse killer T cell line, and a mouse natural killer cell line; induce killer cells in normal mouse spleen cells; and induce antibody forming cells in nude mouse spleen cells. No differences in these biological activities were found between two forms of natural IL-2 that were separable by reverse phase high performance liquid chromatography. |
| Databáze: | OpenAIRE |
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