Immunoreactive cytochrome P-45017 in rat and guineapig gonads, adrenal glands and brain
| Autor: | N. Sananès, M. Gouézou, C. Le Goascogne, Etienne-Emile Baulieu, S. Kominami, S. Takemori, Paul Robel |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Male
endocrine system Embryology medicine.medical_specialty Gonad Guinea Pigs Alpha (ethology) Ovary Biology Chorionic Gonadotropin Endocrinology Pregnancy Internal medicine Testis medicine Animals Sexual Maturation Cellular localization Estradiol Adrenal cortex Brain Leydig Cells Steroid 17-alpha-Hydroxylase Obstetrics and Gynecology Rats Inbred Strains Cell Biology Immunohistochemistry Rats Staining Mifepristone medicine.anatomical_structure Reproductive Medicine Theca Theca Cells Adrenal Cortex Female |
| Zdroj: | Reproduction. 93:609-622 |
| ISSN: | 1741-7899 1470-1626 |
| DOI: | 10.1530/jrf.0.0930609 |
| Popis: | The cytochrome P-450(17 alpha)-hydroxylase, 17----20 lyase (P-450(17 alpha)) is the key enzyme responsible for the biosynthesis of androgens in steroidogenic organs. Its cellular localization has been examined with an immunohistochemical technique. In immature rat ovary, P-450(17 alpha) was first detected in sparse interstitial cells on postnatal Day 8. The number of immunoreactive interstitial cells increased thereafter and the intensity of P-450(17 alpha) staining in these cells was highest at 3 weeks of age. The intensity of staining then started to decline and was very faint at Day 35. From 6 weeks on, the distribution of immunoreactive P-450(17 alpha) was of the adult type: it was detected exclusively in the thecal cells of the large antral, preovulatory, follicles. P-450(17 alpha) was not detectable during pregnancy except on the day of parturition, when thecal cells were transiently immunoreactive. The staining had vanished 24 h after delivery. Human chorionic gonadotrophin (hCG), injected into immature females on Days 24 to 26, induced P-450(17 alpha) prematurely in thecal cells. When injected on Days 12 to 14 of pregnancy, hCG also induced P-450(17 alpha) in the thecal cells surrounding the largest follicles, whereas the interstitial and luteal cells were not immunostained. The antiprogestin RU486, injected on Day 16 of pregnancy, reinstated P-450(17 alpha) (and P-450scc) immunoreactivity in the thecal cells. Oestradiol selectively suppressed P-450(17 alpha) expression in the thecal cells of RU486-treated females. In immature guinea-pig ovary, P-450(17 alpha) was immunostained in thecal cells, not in interstitial cells, although the interstitial cells expressed the delta 5-3 beta-hydroxysteroid dehydrogenase. P-450(17 alpha) was also immunolocalized in the Leydig cells of rat and guinea-pig testes, and in the guinea-pig adrenal cortex (zonae fasciculata and reticularis), but not in the rat adrenal cortex. P-450(17 alpha) was not detectable in the brain of either rat or guinea-pig. |
| Databáze: | OpenAIRE |
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