Modelled microgravity does not modify the yield of chromosome aberrations induced by high-energy protons in human lymphocytes
| Autor: | G.A.P. Cirrone, Paola Scampoli, G. Gialanella, M. Lattuada, Maria Gabriella Sabini, Lorenzo Manti, L.M. Valastro, G. F. Grossi, Marco Durante, Mariagabriella Pugliese |
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| Přispěvatelé: | Manti, Lorenzo, Durante, Marco, Grossi, Gianfranco, M., Lattuada, Pugliese, Mariagabriella, M. G., Sabini, Scampoli, Paola, L., Valastro, Gialanella, Giancarlo |
| Rok vydání: | 2005 |
| Předmět: |
High energy
Cell Survival DNA damage chemistry.chemical_compound Humans Radiology Nuclear Medicine and imaging Lymphocytes Cells Cultured Weightlessness Simulation Cell Proliferation Cell Size Phytohaemagglutinin Chromosome Aberrations Radiological and Ultrasound Technology biology Cell growth Chromosome Dose-Response Relationship Radiation Cell cycle chemistry Yield (chemistry) Immunology biology.protein Biophysics Protons Bromodeoxyuridine |
| Zdroj: | International Journal of Radiation Biology. 81:147-155 |
| ISSN: | 1362-3095 0955-3002 |
| Popis: | The aim was to evaluate the effect of modelled microgravity on radiation-induced chromosome aberrations (CAs). G(0) peripheral blood lymphocytes were exposed to 60 MeV protons or 250 kV(p) X-rays in the dose range 0 - 6 Gy, and allowed to repair DNA damage for 24 h under either normal gravity or microgravity modelled by the NASA-designed rotating-wall bioreactor. Cells were then stimulated to proliferate by phytohaemagglutinin (PHA) under normal gravity conditions and prematurely condensed chromosomes were harvested after 48 h. CAs were scored in chromosomes 1 and 2 by fluorescence in-situ hybridization. Proliferation gravisensitivity was examined by cell growth curves and by morphological evaluation of mitogen-induced activation. Cell replication rounds were monitored by bromodeoxyuridine labelling. Modelled microgravity markedly reduced PHA-mediated lymphocyte blastogenesis and cell growth. However, no significant differences between normal gravity and modelled microgravity were found in the dose - response curves for the induction of aberrant cells or total interchromosomal exchange frequency. Rotating-wall bioreactor-based microgravity reproduced space-related alterations of mitogen stimulation in human lymphocytes but did not affect the yield of CAs induced by low-linear energy transfer radiation. |
| Databáze: | OpenAIRE |
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