Schistosoma mansoni infection decreases IL-33-mRNA expression and increases CXCL9 and CXCL10 production by peripheral blood cells

Autor: Wheverton Ricardo Correia do Nascimento, Cassia Giselle de Oliveira Nóbrega, Erica de Souza Fernandes, Patrícia d’Emery Alves Santos, Fábio Lopes Melo, Mônica Camelo Pessôa de Azevedo Albuquerque, Virgínia Maria Barros de Lorena, Vláudia Maria Assis Costa, Constança Clara Gayoso Simões Barbosa, Valdênia Maria Oliveira de Souza
Rok vydání: 2022
Předmět:
Zdroj: Medical Microbiology and Immunology. 211:211-218
ISSN: 1432-1831
0300-8584
DOI: 10.1007/s00430-022-00745-6
Popis: Schistosoma mansoni infections, particularly egg antigens, induce Th2-dominant granulomatous responses accompanied by remarkable immunoregulatory mechanisms that avoid intense fibrosis. Interleukin (IL)-33 is a cytokine that stimulates the early activation of Th2 responses, and its soluble ST2 receptor (sST2) avoids granulomatous response, as well as CXCL9 and CXCL10 chemokines that have antifibrotic activity. However, in schistosomiasis, these molecules have not been suitably studied. Therefore, this study aimed to measure IL-33 and sST2 RNA, cytokines, and chemokines in peripheral blood cultures from individuals living in schistosomiasis-endemic areas. Peripheral blood cells from individuals with S. mansoni (n = 34) and non-infected individuals (n = 31) were cultured under mitogen stimulation. Supernatant chemokines and cytokines were evaluated using a cytometric bead array, and IL-33 and sST2 mRNA expression was measured using qPCR. Infected individuals showed higher levels of CXCL8, CXCL9, CXCL10, IFN-γ, TNF-α, IL-6, IL-2, IL-4, and IL-10; there was a lower expression of IL-33 mRNA and similar expression of sST2mRNA in infected than non-infected individuals. In conclusion, for the first time, we demonstrated lower IL-33mRNA expression and high levels of the antifibrotic chemokines CXCL9 and CXCL10 in schistosomiasis mansoni, which could control exacerbations of the disease in individuals from endemic areas.
Databáze: OpenAIRE
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