PHOSPHOLIPIDS REACYLATION AND PALMITOYLCOA CONTROL TUMOUR NECROSIS FACTOR-α SENSITIVITY
| Autor: | Pascal George, Pierre Louisot, Catherine Rey, Dominique Ardail, Christiane Levrat |
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| Rok vydání: | 2001 |
| Předmět: |
Time Factors
Membrane permeability Immunology Palmitic Acid Endogeny Biology Biochemistry Linoleic Acid Palmitic acid Mice chemistry.chemical_compound Adenosine Triphosphate Phosphatidylcholine Tumor Cells Cultured Animals Humans Immunology and Allergy Molecular Biology Chromatography High Pressure Liquid Phosphatidylethanolamine Palmitoyl Coenzyme A Tumor Necrosis Factor-alpha Hydrolysis Phosphatidylethanolamines Acetylation Esters Hematology Adenosine Monophosphate Mitochondria Adenosine Diphosphate Microscopy Electron chemistry Permeability (electromagnetism) Cell culture Phosphatidylcholines Acyl Coenzyme A Acyl group Signal Transduction |
| Zdroj: | Cytokine. 13:257-263 |
| ISSN: | 1043-4666 |
| Popis: | From the hypothesis that in TNF-alpha-resistant cells the activity of mitochondrial phospholipase A2 could be reversed by a lysophospholipid acyltransferase, we report that the mitochondrial reacylation of phosphatidylcholine as phosphatidylethanolamine was considerably higher in C6 (TNF-alpha-resistant) than in WEHI-164 (TNF-alpha-sensitive) cells. TNF-alpha did not modify the phospholipids' reacylation in C6, while in WEHI-164 it was increased several-fold. These results suggest that TNF-alpha is not sufficient to restore the barrier permeability in sensitive cells, but may be enough to explain the absence of permeability change in resistant cells. AcylCoA esters, depending on whether the acyl group is unsaturated or saturated (palmitic acid), could control membrane permeability either by participating in the reacylation of phospholipids or keeping the pore in a closed state. The analysis of the endogenous acylCoA ester pools of both cell lines show that the amount of palmitoylCoA is higher in resistant than sensitive cell lines. TNF-alpha treatment does not change these results. |
| Databáze: | OpenAIRE |
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