Fecal microbiota in pouchitis and ulcerative colitis
| Autor: | Litian Wang, Jiang-peng Wei, Ying-Ying Zhang, Gang Liu, Sen-Yang Gao, Kai-Yu Li, Jian-lin Wang |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Faecalibacterium prausnitzii Pouchitis medicine.disease_cause Gastroenterology law.invention 03 medical and health sciences Feces 0302 clinical medicine Intestinal flora law Internal medicine medicine Humans Polymerase chain reaction biology business.industry Case-control study General Medicine Clostridium perfringens Basic Study biology.organism_classification medicine.disease Ulcerative colitis 030104 developmental biology Case-Control Studies Ileal pouch-anal anastomosis 030211 gastroenterology & hepatology Colitis Ulcerative business Temperature gradient gel electrophoresis Disease activity index |
| Zdroj: | World Journal of Gastroenterology |
| ISSN: | 2219-2840 |
| Popis: | AIM To investigate the changes in microbiota in feces of patients with ulcerative colitis (UC) and pouchitis using genomic technology. METHODS Fecal samples were obtained from UC patients with or without an ileal pouch-anal anastomosis (IPAA) procedure, as well as healthy controls. The touchdown polymerase chain reaction technique was used to amplify the whole V3 region of the 16S rRNA gene, which was transcribed from DNA extracted from fecal samples. Denaturing gradient gel electrophoresis was used to separate the amplicons. The band profiles and similarity indices were analyzed digitally. The predominant microbiota in different groups was confirmed by sequencing the 16S rRNA gene. RESULTS Microbial biodiversity in the healthy controls was significantly higher compared with the UC groups (P < 0.001) and IPAA groups (P < 0.001). Compared with healthy controls, the UC patients in remission and those in the mildly active stage, the predominant species in patients with moderately and severely active UC changed obviously. In addition, the proportion of the dominant microbiota, which was negatively correlated with the disease activity of UC (r = -6.591, P < 0.01), was decreased in pouchitis patients. The numbers of two types of bacteria, Faecalibacterium prausnitzii and Eubacterium rectale, were reduced in UC. Patients with pouchitis had an altered microbiota composition compared with UC patients. The microbiota from pouchitis patients was less diverse than that from severely active UC patients. Sequencing results showed that similar microbiota, such as Clostridium perfringens, were shared in both UC and pouchitis. CONCLUSION Less diverse fecal microbiota was present in patients with UC and pouchitis. Increased C. perfringens in feces suggest its role in the exacerbation of UC and pouchitis. |
| Databáze: | OpenAIRE |
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