Silencing of ATP Synthase β Impairs Egg Development in the Leafhopper Scaphoideus titanus, Vector of the Phytoplasma Associated with Grapevine Flavescence Dorée
| Autor: | Ripamonti, Matteo, Cerone, Luca, Abbà, Simona, Rossi, Marika, Ottati, Sara, Palmano, Sabrina, Marzachì, Cristina, Galetto, Luciana |
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| Rok vydání: | 2022 |
| Předmět: |
female sterility
Phytoplasma Cell Survival QH301-705.5 hexamerin Article Catalysis cathepsin L Hemiptera Inorganic Chemistry Euscelidius variegatus Oogenesis RNA interference Animals Vitis Gene Silencing Biology (General) Physical and Theoretical Chemistry QD1-999 Molecular Biology Spectroscopy Plant Diseases RNA Double-Stranded Base Sequence Organic Chemistry Sequence Analysis DNA General Medicine Mitochondrial Proton-Translocating ATPases RNAi specificity Computer Science Applications Chemistry Gene Expression Regulation Female |
| Zdroj: | International Journal of Molecular Sciences International Journal of Molecular Sciences; Volume 23; Issue 2; Pages: 765 International Journal of Molecular Sciences, Vol 23, Iss 765, p 765 (2022) International journal of molecular sciences (Online) 23 (2022). doi:10.3390/ijms23020765 info:cnr-pdr/source/autori:Ripamonti M., Cerone L., Abba S., Rossi M., Ottati S., Palmano S., Marzachi C., Galetto L./titolo:Silencing of ATP Synthase ? Impairs Egg Development in the Leafhopper Scaphoideus titanus, Vector of the Phytoplasma Associated with Grapevine Flavescence Dorée/doi:10.3390%2Fijms23020765/rivista:International journal of molecular sciences (Online)/anno:2022/pagina_da:/pagina_a:/intervallo_pagine:/volume:23 |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms23020765 |
| Popis: | Scaphoideus titanus(Hemiptera:Cicadellidae) is the natural vector of Flavescence dorée phytoplasma, a quarantine pest of grapevine with severe impact on European viticulture. RNA interference (RNAi) machinery components are present inS. titanustranscriptome and injection of ATP synthase β dsRNAs into adults caused gene silencing, starting three days post injection (dpi) up to 20 dpi, leading to decrease cognate protein. Silencing of this gene in the closely related leafhopperEuscelidiusvariegatuspreviously showed female sterility and lack of mature eggs in ovaries. Here, alteration of developing egg morphology inS. titanusovaries as well as overexpression of hexamerin transcript (amino acid storage protein) and cathepsin L protein (lysosome proteinase) were observed in dsATP-injected females. To evaluate RNAi-specificity,E.variegatuswas used as dsRNA-receiving model-species. Different doses of two sets of dsRNA-constructs targeting distinct portions of ATP synthase β gene of both species induced silencing, lack of egg development, and female sterility inE. variegatus, indicating that off-target effects must be evaluated case by case. The effectiveness of RNAi inS. titanusprovides a powerful tool for functional genomics of this non-model species and paves the way toward RNAi-based strategies to limit vector population, despite several technical and regulatory constraints that still need to be overcome to allow open field application. |
| Databáze: | OpenAIRE |
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