Evidence for positive selection and recombination hotspots in Deformed wing virus (DWV)
| Autor: | J. Vallon, Cédric Alaux, B. Moury, Y. Le Conte, Marianne Coulon, C. Desbiez, Maxime Thomasson, Anne Dalmon |
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| Přispěvatelé: | Dalmon, Anne, Abeilles et Environnement (AE), Institut National de la Recherche Agronomique (INRA)-Avignon Université (AU), UMT PrADE, Station de Pathologie Végétale (AVI-PATHO), Institut National de la Recherche Agronomique (INRA), Laboratoire de Sophia Antipolis, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Unité de Pathologie Végétale (PV) |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Untranslated region [SDV.SA]Life Sciences [q-bio]/Agricultural sciences 030106 microbiology Mutation Missense Genome Viral virus Virus Article Evolution Molecular 03 medical and health sciences Complete sequence Viral Proteins varroa destructor Deformed wing virus Animals Point Mutation RNA Viruses Selection Genetic abeille Genetics Recombination Genetic Multidisciplinary biology Whole Genome Sequencing Bees moyen de lutte biology.organism_classification Virology Agricultural sciences 030104 developmental biology Varroa destructor Viral evolution évolution génétique des populations Varroa 5' Untranslated Regions bee Recombination RNA Helicases Sciences agricoles |
| Zdroj: | Scientific Reports 41045 (7), 1-12. (2017) Scientific Reports Scientific Reports, Nature Publishing Group, 2017, 7 (41045), pp.1-12. ⟨10.1038/srep41045⟩ |
| ISSN: | 2045-2322 |
| Popis: | Deformed wing virus (DWV) is considered one of the most damaging pests in honey bees since the spread of its vector, Varroa destructor. In this study, we sequenced the whole genomes of two virus isolates and studied the evolutionary forces that act on DWV genomes. The isolate from a Varroa-tolerant bee colony was characterized by three recombination breakpoints between DWV and the closely related Varroa destructor virus-1 (VDV-1), whereas the variant from the colony using conventional Varroa management was similar to the originally described DWV. From the complete sequence dataset, nine independent DWV-VDV-1 recombination breakpoints were detected, and recombination hotspots were found in the 5′ untranslated region (5′ UTR) and the conserved region encoding the helicase. Partial sequencing of the 5′ UTR and helicase-encoding region in 41 virus isolates suggested that most of the French isolates were recombinants. By applying different methods based on the ratio between non-synonymous (dN) and synonymous (dS) substitution rates, we identified four positions that showed evidence of positive selection. Three of these positions were in the putative leader protein (Lp), and one was in the polymerase. These findings raise the question of the putative role of the Lp in viral evolution. |
| Databáze: | OpenAIRE |
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