Endosulfan affects uterine development and functional differentiation by disrupting Wnt7a and β-catenin expression in rats
| Autor: | Paola I. Ingaramo, Mónica Muñoz-de-Toro, Lucía Vigezzi, Maria Mercedes Milesi, Jorgelina Varayoud, Enrique H. Luque, Marlise Guerrero Schimpf, Jorge G. Ramos |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Cellular differentiation Diethylstilbestrol Uterus Biology Biochemistry 03 medical and health sciences 0302 clinical medicine Endocrinology Proto-Oncogene Proteins Internal medicine medicine Animals Rats Wistar Molecular Biology beta Catenin 030219 obstetrics & reproductive medicine Gene Expression Regulation Developmental Cell Differentiation Embryo Epithelium Rats Wnt Proteins 030104 developmental biology medicine.anatomical_structure WNT7A Animals Newborn Catenin Immunohistochemistry Female Endosulfan medicine.drug |
| Zdroj: | Molecular and Cellular Endocrinology. 425:37-47 |
| ISSN: | 0303-7207 |
| DOI: | 10.1016/j.mce.2016.02.011 |
| Popis: | Neonatal exposure to a low dose of endosulfan may disrupt the expression of Wnt7a and β-catenin during uterine development leading to the failure of uterine functional differentiation during implantation. New-born female Wistar rats were treated with vehicle, endosulfan (600 μg/kg/d, E600) or diethylstilbestrol (0.2 μg/kg/d, DES) on postnatal days (PNDs) 1, 3, 5 and 7. Subsequently, uterine histomorphology and the protein expression of Wnt7a and β-catenin were evaluated on PND8, PND21 and gestational day (GD) 5 (pre-implantation period). In the E600 rats, Wnt7a and β-catenin protein expression was increased in the epithelium on PND8, and Wnt7a expression was decreased in the endometrial glands on PND21. On GD5, the number of uterine glands was decreased in the E600-and DES-treated rats. In addition, Wnt7a expression was decreased in all uterine compartments, and β-catenin expression was increased in the luminal and glandular epithelia of the E600-and DES-treated rats. Disruption of Wnt7a and β-catenin uterine expression in the prepubertal and adult females altered the uterine preparation for embryo implantation, which could be associated with the subfertility triggered by endosulfan. |
| Databáze: | OpenAIRE |
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