Bone marrow mesenchymal stem cells conditioned medium protects VSC4.1 cells against 2,5-hexanedione-induced autophagy via NGF-PI3K/Akt/mTOR signaling pathway
| Autor: | Yijie Sun, Zhiqiang Qian, Xiaoxia Shi, Xin Zhang, Ying Kong, Yong-Jun Piao, Chenxue Gao, Shuangyue Li, Fengyuan Piao |
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| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Cell Survival Apoptosis Bone Marrow Cells Rats Sprague-Dawley Phosphatidylinositol 3-Kinases 03 medical and health sciences chemistry.chemical_compound Nerve Growth Factor Autophagy medicine Animals Viability assay Molecular Biology Cell damage Protein kinase B PI3K/AKT/mTOR pathway Sirolimus Chemistry TOR Serine-Threonine Kinases General Neuroscience Mesenchymal stem cell Mesenchymal Stem Cells medicine.disease Rats Cell biology Hexanones Neuroprotective Agents 030104 developmental biology medicine.anatomical_structure Culture Media Conditioned Neurology (clinical) Bone marrow K252a Proto-Oncogene Proteins c-akt Signal Transduction Developmental Biology |
| Zdroj: | Brain Research. 1696:1-9 |
| ISSN: | 0006-8993 |
| DOI: | 10.1016/j.brainres.2018.04.028 |
| Popis: | We aimed to investigate the effects of bone marrow mesenchymal stem cell conditioned medium (BMSC-CM) in preventing 2,5-hexanedione (HD)-induced damage to motoneurons, and examined the molecular mechanisms that mediate these effects. VSC4.1 cells were exposed to 25 mM HD for 24 h followed by incubation with DMEM for 24 h. HD-treated cells were incubated with BMSC-CM at varied concentrations. Incubation with BMSC-CM ameliorated the decreased cell viability and reduced LDH release from cells exposed to HD. BMSC-CM suppressed the elevated number of autophagic vacuoles, cells with LC3 puncta, increased LC3-II/LC3-I ratio, and decreased p62 caused by HD exposure. BMSC-CM elevated NGF and p-TrkA expressions in HD-treated cells. Administration of NGF inhibited autophagy, an effect that was similar to that observed after BMSC-CM treatment; this effect was abolished by the addition of NGF-neutralizing antibodies. BMSC-CM or NGF elevated p-protein kinase B (Akt) and p-mammalian target of rapamycin (mTOR) in HD-exposed cells, which was interrupted by TrkA inhibitor, K252a and mTOR inhibitor, rapamycin. BMSC-CM prevented HD-induced autophagic cell damage in VSC4.1 cells. The neuroprotective effect of BMSC-CM appeared to be at least partly associated with its ability to trigger the NGF-phosphatidylinositol-3-kinase (PI3K)/Akt/mTOR signaling pathway. |
| Databáze: | OpenAIRE |
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