Expression of tryptophan hydroxylase in gastric mucosa in symptomatic and asymptomatic Helicobacter pylori infection
| Autor: | Jan Chojnacki, Marek Romanowski, Cezary Chojnacki, Tomasz Poplawski, Aleksandra Błońska, Janusz Blasiak |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
medicine.medical_specialty
Urea breath test Epigastric pain Gastroenterology Asymptomatic Pathogenesis 03 medical and health sciences 0302 clinical medicine Clinical Research Internal medicine medicine Gastric mucosa gastric mucosa 030212 general & internal medicine Antrum biology medicine.diagnostic_test business.industry chronic dyspepsia General Medicine Helicobacter pylori Tryptophan hydroxylase biology.organism_classification increased expression of tph-1 in gastric mucosa plays a role in pathogenesis of chronic dyspepsia medicine.anatomical_structure helicobacter pylori Medicine medicine.symptom business tryptophan hydroxylase |
| Zdroj: | Archives of Medical Science, Vol 15, Iss 2, Pp 416-423 (2019) Archives of Medical Science : AMS |
| ISSN: | 1896-9151 1734-1922 |
| Popis: | Introduction Helicobacter pylori infection induces clinical symptoms in 15–20% of subjects, and the reason for this variation is still not clear. The aim of the present study is to evaluate the expression of tryptophan hydroxylase (TpH-1) in gastric mucosa of patients with symptomatic and asymptomatic H. pylori infection in relation to the intensity of bacterial colonization and severity of dyspeptic symptoms. Material and methods Ninety subjects (aged 35–49 years) were enrolled in the study and separated into 3 groups of 30 subjects each. Group I – healthy volunteers without H. pylori infection, group II – subjects with asymptomatic H. pylori infection, group III – H. pylori-infected patients with dyspeptic symptoms, mainly fasting and nocturnal epigastric pain. To diagnose H. pylori infection the urea breath test (UBT-13C) and histological analysis were performed. The level of mRNA expression of tryptophan hydroxylase (TpH-1) was estimated in gastric mucosa with RT-PCR. Results The expression of this enzyme in antral mucosa was 2.69 ±0.97 in group I, 2.28 ±0.69 in group II (p > 0.05) and 4.40 ±1.64 in group III (p < 0.001). The levels of expression of TpH-1 in gastric body mucosa were 2.16 ±0.70, 1.57 ±0.52 (p > 0.05) and 3.40 ±1.51 (p < 0.001), respectively. In group III a positive correlation was found between intensity of H. pylori colonization and TpH-1 expression as well as between TpH-1 expression and severity of dyspeptic symptoms. Conclusions Increased expression of TpH-1 in gastric mucosa plays a role in pathogenesis of chronic dyspepsia. |
| Databáze: | OpenAIRE |
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