Purification and Characterization of the Reconstitutively Active Adenine Nucleotide Carrier from Maize Mitochondria
| Autor: | A. De Santis, Carmela Ponzone, Luisa Stefanizzi, G. Genchi, Ferdinando Palmieri, Faustino Bisaccia |
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| Rok vydání: | 1996 |
| Předmět: |
GTP'
Physiology Molecular Sequence Data Plant Science Mitochondrion Biology Zea mays chemistry.chemical_compound Adenosine Triphosphate Adenine nucleotide Genetics Cardiolipin Amino Acid Sequence Pyridoxal chemistry.chemical_classification Chromatography Molecular mass Membrane Proteins Biological Transport Guanine Nucleotides Mersalyl Mitochondria Amino acid Adenosine Diphosphate Kinetics chemistry Biochemistry Liposomes Carrier Proteins Mitochondrial ADP ATP Translocases Sequence Analysis Plant Shoots Research Article |
| Zdroj: | Plant Physiology. 112:845-851 |
| ISSN: | 1532-2548 0032-0889 |
| Popis: | The adenine nucleotide carrier from maize (Zea mays L. cv B 73) shoot mitochondria was solubilized with Triton X-100 and purified by sequential chromatography on hydroxyapatite and Matrex Gel Blue B in the presence of cardiolipin and asolectin. Sodium dodecyl sulfate-gel electrophoresis of the purified fraction showed a single polypeptide band with an apparent molecular mass of 32 kD. When reconstituted in liposomes, the adenine nucleotide carrier catalyzed a pyridoxal 5[prime]-phosphate-sensitive ATP/ATP exchange. It was purified 168-fold with a recovery of 60% and a protein yield of 0.25% with respect to the mitochondrial extract. Among the various substrates and inhibitors tested, the reconstituted protein transported only ADP, ATP, GDP, and GTP, and was inhibited by atractyloside, bongkrekate, phenylisothiocianate, pyridoxal 5[prime]-phosphate, and mersalyl (but not N-ethylmaleimide). Maximum initial velocity of the reconstituted ATP/ATP exchange was determined to be 2.2 [mu]mol min-1 mg-1 protein at 25[deg]C. The half-saturation constants and the corresponding inhibition constants were 17 [mu]M for ATP, 26 [mu]M for ADP, 59 [mu]M for GTP, and 125 [mu]M for GDP. The activation energy of the ATP/ATP exchange was 48 kilojoule/mol between 0 and 15[deg]C, and 22 kilojoule/mol between 15 and 35[deg]C. Partial amino acid sequences showed that the purified protein was the product of the ANT-G1 gene sequenced previously (B. Bathgate, A. Baker, C.J. Leaver [1989] Eur J Biochem 183: 303–310). |
| Databáze: | OpenAIRE |
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