Arbitrarily primed PCR to type Vibrio spp. pathogenic for shrimp
| Autor: | Fabrice Merien, Isabelle Mermoud, Philippe Perolat, Cyrille Goarant, Franck Berthe |
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| Přispěvatelé: | Ifremer - Nouvelle-Calédonie, Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Institut Pasteur de Nouvelle-Calédonie, Réseau International des Instituts Pasteur (RIIP), Laboratoire Génétique et Pathologie (IFREMER- LGP), Département Elevage et médecine vétérinaire (Cirad-EMVT), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), This work was partly supported by grants from the South and North Provinces of New Caledonia. V. penaeicida KH-1Tand the three Japanese field isolates were kindly provided by K. Muroga (Faculty of Applied Biological Science, Hiroshima University, Higashi-Hiroshima, Japan). F.M. and P.P. were supported by the International Network of Pasteur Institutes (General Delegation, Institute Pasteur, Paris, France)., Thanks are due to Vincent Pomarède for technical assistance, to Eric Boglio for editorial advice, and the Territorial Laboratory of Veterinary Diagnosis of New Caledonia for maintaining and supplying the Vibrio field isolate collection. |
| Jazyk: | angličtina |
| Rok vydání: | 1999 |
| Předmět: |
Population
Zoology Aquaculture Applied Microbiology and Biotechnology Polymerase Chain Reaction MESH: Bacterial Typing Techniques Microbiology Disease Outbreaks 03 medical and health sciences New Caledonia MESH: Vibrio Vibrionaceae [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases Decapoda MESH: DNA Fingerprinting Animals MESH: Animals 14. Life underwater Typing MESH: Aquaculture MESH: Disease Outbreaks education 030304 developmental biology Vibrio 0303 health sciences education.field_of_study Ecology biology 030306 microbiology Outbreak MESH: Polymerase Chain Reaction biology.organism_classification MESH: Decapoda (Crustacea) MESH: New Caledonia DNA Fingerprinting Shrimp Bacterial Typing Techniques Environmental and Public Health Microbiology DNA profiling Prawn Food Science Biotechnology |
| Zdroj: | Applied and Environmental Microbiology Applied and Environmental Microbiology, American Society for Microbiology, 1999, 65 (3), pp.1145-1151 Applied And Environmental Microbiology (0099-2240) (Amer Soc Microbiology), 1999-03, Vol. 65, N. 3, P. 1145-1151 Scopus-Elsevier |
| ISSN: | 0099-2240 1098-5336 |
| Popis: | A molecular typing study on Vibrio strains implicated in shrimp disease outbreaks in New Caledonia and Japan was conducted by using AP-PCR (arbitrarily primed PCR). It allowed rapid identification of isolates at the genospecies level and studies of infraspecific population structures of epidemiological interest. Clusters identified within the species Vibrio penaeicida were related to their area of origin, allowing discrimination between Japanese and New Caledonian isolates, as well as between those from two different bays in New Caledonia separated by only 50 km. Other subclusters of New Caledonian V. penaeicida isolates could be identified, but it was not possible to link those differences to accurate epidemiological features. This contribution of AP-PCR to the study of vibriosis in penaeid shrimps demonstrates its high discriminating power and the relevance of the epidemiological information provided. This approach would contribute to better knowledge of the ecology of Vibrio spp. and their implication in shrimp disease in aquaculture. |
| Databáze: | OpenAIRE |
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