Integrating molecularly imprinted polymer beads in graphite-epoxy electrodes for the voltammetric biosensing of histamine in wines
| Autor: | Manel del Valle, Anna Herrera-Chacon, Şule Dinç-Zor |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Polymers
Biogenic amines Wine Biosensing Techniques 02 engineering and technology 01 natural sciences Analytical Chemistry Molecular Imprinting Limit of Detection Adsorptive stripping voltammetry Electrodes Voltammetry chemistry.chemical_classification Detection limit Molecularly imprinted polymer Chromatography Epoxy Resins Sensors 010401 analytical chemistry Electrochemical Techniques Polymer 021001 nanoscience & nanotechnology 0104 chemical sciences chemistry Electrode Precipitation polymerization Graphite 0210 nano-technology Biosensor Histamine |
| Zdroj: | Dipòsit Digital de Documents de la UAB Universitat Autònoma de Barcelona |
| Popis: | This manuscript presents a voltammetric biosensing study with use of molecularly imprinted polymers to detect histamine in wine. Polymer beads were synthesized by standard precipitation polymerization method and implemented on the electrode surface via sol-gel immobilization. Scanning and confocal microscopy examinations permitted characterizing the material. Adsorptive stripping voltammetry in differential mode was the technique chosen for final application, selecting an enrichment time of 5 min. These conditions permitted a limit of detection of 0.19 μg mL−1 (1.0 μM), with a linear response range from 0.5 to 6.0 μg mL−1 (2.71–32.4 μM). The repeatability of the measurements was 4.6% relative standard deviation (n = 12). Principal component analysis showed the ability of the prepared receptor for discriminating other biogenic amines and potential interfering species. A final application, illustrating the determination of histamine, was completed to show agreement of results between the fluorimetric reference method and the proposed electrochemical approach. |
| Databáze: | OpenAIRE |
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