Encapsulation of Doxorubicin in Furcellaran/Chitosan Nanocapsules by Layer-by-Layer Technique for Selectively Controlled Drug Delivery
| Autor: | Zbynek Heger, Vedran Milosavljevic, Hana Michalkova, Milica Gagic, Lukas Richtera, Pavel Kopel, Amitava Moulick, Yazan Haddad, Radka Balkova, Ewelina Jamróz, Barbora Tesarova, Vojtech Adam |
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| Rok vydání: | 2019 |
| Předmět: |
Polymers and Plastics
Alginates DNA damage media_common.quotation_subject Bioengineering 02 engineering and technology 010402 general chemistry Endocytosis Hemolysis 01 natural sciences Nanocapsules Biomaterials Chitosan chemistry.chemical_compound Drug Delivery Systems Cell Line Tumor Plant Gums Toxicity Tests Materials Chemistry medicine Humans Doxorubicin Internalization media_common Peptide modification Hydrogen-Ion Concentration Middle Aged 021001 nanoscience & nanotechnology Polyelectrolytes 0104 chemical sciences Platelet Endothelial Cell Adhesion Molecule-1 Drug Liberation HEK293 Cells chemistry Delayed-Action Preparations Drug delivery Biophysics Female Peptides 0210 nano-technology medicine.drug |
| Zdroj: | Biomacromolecules. 21:418-434 |
| ISSN: | 1526-4602 1525-7797 |
| Popis: | Minimization of drug side effects is a hallmark of advanced targeted therapy. Herein we describe the synthesis of polysaccharide-based nanocapsules prepared from furcellaran and chitosan via layer-by-layer deposition using electrostatic interaction. Using doxorubicin as a model drug, prepared nanocapsules showed excellent drug loading properties and release influence by pH and stability. Targeted delivery of doxorubicin was achieved by nanocapsule surface modification using homing peptide (seq SMSIARLC). The synthesized nanocapsules possess excellent compatibility to eukaryotic organisms. In the case of nonmalignant cells (PNT1A and HEK-293), toxicity tests revealed the absences of DNA fragmentation, apoptosis, necrosis, and also disruption of erythrocyte membranes. In contrast, results from treatment of malignant cell lines (MDA-MB-231 and PC3) indicate good anticancer effects of synthesized bionanomaterial. Internalization studies revealed the nanocapsule's ability to enter the malignant cell lines by endocytosis and triggering the apoptosis. The occurrence of apoptosis is mostly connected to the presence of ROS and inability of DNA damage reparation. Additionally, the obtained results strongly indicate that peptide modification increases the speed of nanocapsule internalization into malignant cell lines while simultaneously nonmalignant cell lines are untouched by nanocapsules highlighting the strong selectivity of the peptide. |
| Databáze: | OpenAIRE |
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