Bisphenol A Analogues Suppress Spheroid Attachment on Human Endometrial Epithelial Cells through Modulation of Steroid Hormone Receptors Signaling Pathway
| Autor: | Sudini R. Fernando, Suranga P. Kodithuwakku, Luhan Jiang, Kai-Fai Lee, William S.B. Yeung, Ernest Hung Yu Ng, Ziyi Wang, Chris K C Wong, Hongjie Fan |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
endocrine system
steroid hormones Steroid hormone receptor Bisphenol Cell Survival QH301-705.5 medicine.medical_treatment Receptors Cell Surface Response Elements Article chemistry.chemical_compound Phenols Genes Reporter Cell Line Tumor Spheroids Cellular medicine Cell Adhesion Humans Sulfones Benzhydryl Compounds endometrium Biology (General) Receptor Cell Proliferation Chemistry urogenital system Epithelial Cells General Medicine co-culture Cell biology Up-Regulation Steroid hormone Endocrine disruptor Bisphenol S spheroid attachment bisphenols Female Signal transduction Transcriptome Estrogen receptor alpha microarray hormones hormone substitutes and hormone antagonists |
| Zdroj: | Cells, Vol 10, Iss 2882, p 2882 (2021) Cells Volume 10 Issue 11 |
| ISSN: | 2073-4409 |
| Popis: | Bisphenol A (BPA) is a well-known endocrine disruptor, widely used in various consumer products and ubiquitously found in air, water, food, dust, and sewage leachates. Recently, several countries have restricted the use of BPA and replaced them with bisphenol S (BPS) and bisphenol F (BPF), which have a similar chemical structure to BPA. Compared to BPA, both BPS and BPF have weaker estrogenic effects, but their effects on human reproductive function including endometrial receptivity and embryo implantation still remain largely unknown. We used an in vitro spheroid (blastocyst surrogate) co-culture assay to investigate the effects of BPA, BPS, and BPF on spheroid attachment on human endometrial epithelial cells, and further delineated their role on steroid hormone receptor expression. We also used transcriptomics to investigate the effects of BPA, BPS, and BPF on the transcriptome of human endometrial cells. We found that bisphenol treatment in human endometrial Ishikawa cells altered estrogen receptor alpha (ERα) signaling and upregulated progesterone receptors (PR). Bisphenols suppressed spheroid attachment onto Ishikawa cells, which was reversed by the downregulation of PR through PR siRNA. Overall, we found that bisphenol compounds can affect human endometrial epithelial cell receptivity through the modulation of steroid hormone receptor function leading to impaired embryo implantation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|