In vivo and in silico studies of Dennettia tripetala essential oil reveal the potential harmful effects of habitual consumption of the plant seed
| Autor: | Patience Boluwatife Okwuese, Moses A. Akanmu, Michael Oluwatoyin Daniyan, Idris Ajayi Oyemitan, V.O. Ekundina, Temilade Feyi Adeyipo |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
PDW
platelet distribution width Health Toxicology and Mutagenesis In silico TG test guidelines MCH mean corpuscular hemoglobin Histopathology MCHC mean corpuscular hemoglobin concentration AST aspartate aminotransferase Biology Pharmacology Toxicology MCV mean corpuscular volume Biochemical law.invention chemistry.chemical_compound Linalool WBC white blood count law In vivo HCT hematocrit RA1190-1270 OECD Organization for Economic Co-operation and Development ALT alanine aminotransferase medicine MPV mean platelet volume Essential oil Hb hemoglobin concentration ComputingMethodologies_COMPUTERGRAPHICS FOB functional observatory batteries Kidney RBC red blood count Dennettia tripetala Humulene Toxicity PLT platelets Regular Article biology.organism_classification medicine.anatomical_structure chemistry RDW-CV and RDW-SD red blood cell distribution width variation coefficient and standard deviation respectively Annonaceae Toxicology. Poisons PCT plateletcrit Haematology |
| Zdroj: | Toxicology Reports Toxicology Reports, Vol 8, Iss, Pp 1488-1497 (2021) |
| ISSN: | 2214-7500 |
| Popis: | Graphical abstract Highlights • Dennettia tripetala seeds are widely consumed for leisure and medicinal purposes. • Orally administered essential oil of the seed altered weights and weight ratios. • Single and repeated oral administration altered hematological and biochemical indices. • Single and repeated oral administration altered histoarchitectures of liver and kidney. • In silico profiling confirm toxicity potential of essential oil of seed of D. tripetala. Dennettia tripetala G. Baker (Annonaceae), is a plant with nutritional, social economy, and medicinal values. Its rising medicinal profile makes this plant a prospect in drug discovery. However, the reported strong addictive potential among habitual consumers makes the need to establish its safety imperative. In this report, we evaluated the safety profile of the essential oil of the seed of D. tripetala (EODS) in nulliparous female Wistar rats using in vivo single and repeated dose toxicity profiling, as well as in silico toxicity profiling of its known seed oil derived phytoconstituents. Our results showed consistent significant dose-dependent alterations in relative body weights, organ-body and organ-brain weight ratios, haematological and biochemical indices, as well as liver and kidney histoarchitectures, following single and repeated oral administrations. Significant alterations in liver and kidney histoarchitectures were consistent with the observed significant increase in AST/ALT ratio, suggesting deleterious effects of EODS on the kidney and liver. However, the lack of alterations in the histoarchitectures of the hippocampus and hypothalamus suggests that the brain may not have been adversely affected. Also, the in silico analysis suggests that hepatotoxic effects of EODS may be linked to Benzylnitrile, Humulene, Linalool, (Z)-ß-Ocimene. In addition, the failure of ß-Phenylnitroethane, the most abundant phytoconstituent of EODS, to pass phases I and II in silico toxicity screening, and the presence of Caryophyllene oxide, a known toxic compound, coupled with the predicted binding of both to DNA and protein, low LD50 and high percent mortality at 250 mg/kg of repeated doses, further confirmed the potentially toxic nature of EODS. We concluded that based on our in vivo and in silico observations, there is an urgent need for public education to regulate the excessive consumption of the seeds of D. tripetala. |
| Databáze: | OpenAIRE |
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