Significance of caveolin-1 and matrix metalloproteinase 14 gene expression in canine mammary tumours
| Autor: | Masashi Ebisawa, Y Endou, Takahiro Komatsu, Y Tochigi, Tsuyoshi Kadosawa, Kazuko Hirayama, Hiroshi Yokota, Miyu Nishikawa, Hidetomo Iwano, Hiroyuki Taniyama |
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| Rok vydání: | 2015 |
| Předmět: |
congenital
hereditary and neonatal diseases and abnormalities Pathology medicine.medical_specialty Caveolin 1 Breast Neoplasms Mammary Neoplasms Animal Matrix metalloproteinase Biology Malignancy Gene Expression Regulation Enzymologic Dogs Cell Line Tumor Gene expression Matrix Metalloproteinase 14 medicine Animals Humans Dog Diseases Gene General Veterinary medicine.disease nervous system diseases Gene Expression Regulation Neoplastic Cell culture Case-Control Studies Invadopodia cardiovascular system Cancer research MMP14 Female Animal Science and Zoology |
| Zdroj: | The Veterinary Journal. 206:191-196 |
| ISSN: | 1090-0233 |
| Popis: | Canine mammary tumours (CMTs) are the most common neoplasms affecting female dogs. There is an urgent need for molecular biomarkers that can detect early stages of the disease in order to improve accuracy of CMT diagnosis. The aim of this study was to examine whether caveolin-1 (Cav-1) and matrix metalloproteinase 14 (MMP14) are associated with CMT histological malignancy and invasion. Sixty-five benign and malignant CMT samples and six normal canine mammary glands were analysed using quantitative reverse transcription-polymerase chain reaction. Cav-1 and MMP14 genes were highly expressed in CMT tissues compared to normal tissues. Cav-1 especially was overexpressed in malignant and invasive CMT tissues. When a CMT cell line was cultured on fluorescent gelatin-coated coverslips, localisation of Cav-1 was observed at invadopodia-mediated degradation sites of the gelatin matrix. These findings suggest that Cav-1 may be involved in CMT invasion and that the markers may be useful for estimating CMT malignancy. |
| Databáze: | OpenAIRE |
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