Repair of critical sized cranial defects with BMP9-transduced calvarial cells delivered in a thermoresponsive scaffold

Autor: Junyi Liao, Viktor Tollemar, Minpeng Lu, Yunxiao Zhu, Russell R. Reid, Tong-Chuan He, Jixing Ye, Guillermo A. Ameer, Zari P. Dumanian
Rok vydání: 2017
Předmět:
0301 basic medicine
Pathology
Scaffold
Physiology
Organogenesis
lcsh:Medicine
Ossification
Polyethylene Glycols
Diagnostic Radiology
Mice
Transduction
Genetic
Animal Cells
Animal Products
Growth Differentiation Factor 2
Medicine and Health Sciences
lcsh:Science
Fracture Healing
Multidisciplinary
Tissue Scaffolds
Chemistry
Stem Cells
Radiology and Imaging
Agriculture
Bone Imaging
Growth Differentiation Factors
Bone Remodeling
Anatomy
Cellular Types
Stem cell
Research Article
Cell Physiology
medicine.medical_specialty
Histology
Mature Bone
Imaging Techniques
Context (language use)
Research and Analysis Methods
Osseointegration
Cell Line
03 medical and health sciences
Diagnostic Medicine
In vivo
Tissue Repair
medicine
Animals
Humans
Progenitor cell
Bone Development
lcsh:R
Skull
Mesenchymal stem cell
Biology and Life Sciences
Mesenchymal Stem Cells
Cell Biology
Surgery
030104 developmental biology
Gelatin
lcsh:Q
Cell Immortalization
Physiological Processes
Organism Development
Developmental Biology
Zdroj: PLoS ONE
PLoS ONE, Vol 12, Iss 3, p e0172327 (2017)
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0172327
Popis: Large skeletal defects caused by trauma, congenital malformations, and post-oncologic resections of the calvarium present major challenges to the reconstructive surgeon. We previously identified BMP-9 as the most osteogenic BMP in vitro and in vivo. Here we sought to investigate the bone regenerative capacity of murine-derived calvarial mesenchymal progenitor cells (iCALs) transduced by BMP-9 in the context of healing critical-sized calvarial defects. To accomplish this, the transduced cells were delivered to the defect site within a thermoresponsive biodegradable scaffold consisting of poly(polyethylene glycol citrate-co-N-isopropylacrylamide mixed with gelatin (PPCN-g). A total of three treatment arms were evaluated: PPCN-g alone, PPCN-g seeded with iCALs expressing GFP, and PPCN-g seeded with iCALs expressing BMP-9. Defects treated only with PPCN-g scaffold did not statistically change in size when evaluated at eight weeks postoperatively (p = 0.72). Conversely, both animal groups treated with iCALs showed significant reductions in defect size after 12 weeks of follow-up (BMP9-treated: p = 0.0025; GFP-treated: p = 0.0042). However, H&E and trichrome staining revealed more complete osseointegration and mature bone formation only in the BMP9-treated group. These results suggest that BMP9-transduced iCALs seeded in a PPCN-g thermoresponsive scaffold is capable of inducing bone formation in vivo and is an effective means of creating tissue engineered bone for critical sized defects.
Databáze: OpenAIRE
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