Thermosensitive Nanospheres with a Gold Layer Revealed as Low-Cytotoxic Drug Vehicles
| Autor: | Mamoun Muhammed, Jong Eun Ihm, Yun Suk Jo, Do Kyung Kim, Jian Qin |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Male
Time Factors Cell Survival Uterine Cervical Neoplasms Antineoplastic Agents Lower critical solution temperature Polyethylene Glycols HeLa Chlorocebus aethiops Polymer chemistry Electrochemistry medicine Animals Humans General Materials Science Viability assay Solubility Fibroblast Cytotoxicity Spectroscopy Acrylamides Drug Carriers Calorimetry Differential Scanning biology Chemistry Temperature Biological Transport Serum Albumin Bovine Surfaces and Interfaces Condensed Matter Physics biology.organism_classification Fluorescence medicine.anatomical_structure Cell culture COS Cells Lactates Female Spectrophotometry Ultraviolet Gold HeLa Cells Nuclear chemistry |
| Zdroj: | Langmuir. 21:9346-9351 |
| ISSN: | 1520-5827 0743-7463 |
| DOI: | 10.1021/la051069t |
| Popis: | In this paper, the positive effect of a gold layer on cell viability is demonstrated by examining the results given by 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfop henyl)-2H-tetrazolium (MTS) assay and two-color cell fluorescence viability (TCCV) assay. These cytotoxicity tests were performed with human cervical adenocarcinoma cells (HeLa cell line) and transformed African green monkey kidney fibroblast cells (Cos-7 cell line). To fabricate the nanostructures as drug vehicles, first, poly(l,l-lactide-co-ethylene glycol) (PLLA-PEG) and poly(N-isopropylacrylamide-co-D,D-lactide) (PNIPAAm-PDLA) were synthesized, and then two kinds of thermosensitive nanospheres comprising "shell-in-shell" structures without a gold layer (PLLA-PEG@PNIPAAm-PDLA) and with a gold layer (Au@PLLA-PEG@PNIPAAm-PDLA) were constructed by a modified double-emulsion method (MDEM). Both of them displayed a unique thermosensitive character exhibiting the lower critical solubility temperature (LCST) at 36.7 degrees C which was confirmed by UV-vis spectroscopy and differential scanning calorimetry (DSC). The release profiles of entrapped bovine serum albumin (BSA) were monitored at 22 and 37 degrees C, respectively, to reveal the thermal dependence on the release rate. In cell viability tests, both PLLA-PEG@PNIPAAm-PDLA and Au@PLLA-PEG@PNIPAAm-PDLA showed excellent cell viability, and furthermore, Au@PLLA-PEG@PNIPAAm-PDLA, particularly at high doses, exhibited more enhanced cell viability than PLLA-PEG@PNIPAAm-PDLA. This effect is mainly attributed to the gold layer which binds the protein molecules first and consequently facilitates transmembrane uptake of essential nutrients in the cell media, resulting in favorable cell proliferation. |
| Databáze: | OpenAIRE |
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