Linking lignocellulosic dietary patterns with gut microbial Enterotypes of Tsaitermes ampliceps and comparison with Mironasutitermes shangchengensis
| Autor: | Liu Yq, Li Yongtao, Hao Lin, Fengqin Wang, Andong Song, Hongying Liu, Su Lijuan, Y.Y. Wang |
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| Rok vydání: | 2015 |
| Předmět: |
biology
Library Firmicutes Bacteroidetes Biodiversity Isoptera Sequence Analysis DNA General Medicine Gut flora biology.organism_classification Animal Feed Lignin Gastrointestinal Microbiome Symbiosis RNA Ribosomal 16S Botany Genetics Animals Cluster Analysis Metagenome Enterotype Food science Proteobacteria Molecular Biology Temperature gradient gel electrophoresis |
| Zdroj: | Genetics and Molecular Research. 14:13954-13967 |
| ISSN: | 1676-5680 |
| Popis: | Tsaitermes ampliceps (lower termites) and Mironasutitermes shangchengensis (higher termites) are highly eusocial insects that thrive on recalcitrant lignocellulosic diets through nutritional symbioses with gut dwelling prokaryotes and eukaryotes. We used denaturing gradient gel electrophoresis and a 16S rRNA clone library to investigate i) how microbial communities adapt to lignocellulosic diets with different cellulose and lignin content, ii) the differences in the dominant gut microbial communities of the 2 types of termites. The results indicated that gut microbiota composition in T. ampliceps was profoundly affected by 2-week diet shifts. Comparison of these changes indicated that Bacteroidetes and Spirochaetes act in cellulose degradation, while Firmicutes were responsible for lignin degradation. Additionally, Proteobacteria consistently participated in energy production and balanced the gut environment. Bacteroidetes may function without hindgut protozoans in higher termites. The diversity of enteric microorganisms in M. shangchengensis was higher than that in T. ampliceps, possibly because of the more complicated survival mechanisms of higher termites. |
| Databáze: | OpenAIRE |
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