In-cell architecture of an actively transcribing-translating expressome

Autor:	Neil Singh, Cedric Blötz, Wim J. H. Hagen, Francis J. O’Reilly, Ludwig Sinn, Swantje Lenz, Jörg Stülke, Andrea Graziadei, Patrick Cramer, Julia Mahamid, Liang Xue, Dimitry Tegunov, Juri Rappsilber
Rok vydání:	2020
Předmět:	Transcription elongation Transcription Genetic Cell Peptide Chain Elongation Translational Review Article Ribosome Genome Transcriptome 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Transcription (linguistics) Bacterial Proteins RNA polymerase medicine Humans Protein Interaction Maps Structural approach 030304 developmental biology 0303 health sciences Multidisciplinary Chemistry DNA-Directed RNA Polymerases Peptide Elongation Factors Molecular machine Cell biology Mycoplasma pneumoniae medicine.anatomical_structure Structural biology Ribosomes 030217 neurology & neurosurgery Genome Bacterial
Zdroj:	Fac Rev O'Reilly, F J, Xue, L, Graziadei, A, Sinn, L, Lenz, S, Tegunov, D, Blötz, C, Singh, N, Hagen, W J H, Cramer, P, Stülke, J, Mahamid, J & Rappsilber, J 2020, ' In-cell architecture of an actively transcribing-translating expressome ', Science, vol. 369, no. 6503, pp. 554-557 . https://doi.org/10.1126/science.abb3758 Science bioRxiv
ISSN:	1095-9203
Popis:	Although much is known about the machinery that executes fundamental processes of gene expression in cells, much also remains to be learned about how that machinery works. A recent paper by O’Reilly et al. reports a major step forward in the direct visualization of central dogma processes at submolecular resolution inside bacterial cells frozen in a native state. The essential methodologies involved are cross-linking mass spectrometry (CLMS) and cryo-electron tomography (cryo-ET). In-cell CLMS provides in vivo protein interaction maps. Cryo-ET allows visualization of macromolecular complexes in their native environment. These methods have been integrated by O’Reilly et al. to describe a dynamic assembly in situ between a transcribing RNA polymerase (RNAP) and a translating ribosome – a complex known as the expressome – in the model bacterium Mycoplasma pneumoniae(1). With the application of improved data processing and classification capabilities, this approach has allowed unprecedented insights into the architecture of this molecular assembly line, confirming the existence of a physical link between RNAP and the ribosome and identifying the transcription factor NusA as the linking molecule, as well as making it possible to see the structural effects of drugs that inhibit either transcription or translation. The work provides a glimpse into the future of integrative structural cell biology and can serve as a roadmap for the study of other molecular machineries in their native context.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d1566ce803de4b7ca020222713aa49e3 https://pubmed.ncbi.nlm.nih.gov/35146496 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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