The ATP/DNA ratio is a better indicator of islet cell viability than the ADP/ATP ratio
| Autor: | Johanna Sotiris, G. M. Wildey, Ali Naji, Klearchos K. Papas, K. Stewart Maynard, Bernhard J. Hering, Thomas M. Suszynski, Eric J. Falde, N. Ko, Gary W. Cline |
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| Rok vydání: | 2008 |
| Předmět: |
Spectrometry
Mass Electrospray Ionization Hot Temperature Cell Survival Cell Culture Techniques Biology Article chemistry.chemical_compound Islets of Langerhans Adenosine Triphosphate Humans Insulin Viability assay Transplantation geography geography.geographical_feature_category Reproducibility of Results DNA Islet Cell biology Adenosine Diphosphate Adenosine diphosphate chemistry Biochemistry Cell culture Surgery ATP–ADP translocase Adenosine triphosphate Intracellular |
| Zdroj: | Transplantation proceedings. 40(2) |
| ISSN: | 0041-1345 |
| Popis: | Real-time, accurate assessment of islet viability is critical for avoiding transplantation of nontherapeutic preparations. Measurements of the intracellular ADP/ATP ratio have been recently proposed as useful prospective estimates of islet cell viability and potency. However, dead cells may be rapidly depleted of both ATP and ADP, which would render the ratio incapable of accounting for dead cells. Since the DNA of dead cells is expected to remain stable over prolonged periods of time (days), we hypothesized that use of the ATP/DNA ratio would take into account dead cells and may be a better indicator of islet cell viability than the ADP/ATP ratio. We tested this hypothesis using mixtures of healthy and lethally heat-treated (HT) rat insulinoma cells and human islets. Measurements of ATP/DNA and ADP/ATP from the known mixtures of healthy and HT cells and islets were used to evaluate how well these parameters correlated with viability. The results indicated that ATP and ADP were rapidly (within 1 hour) depleted in HT cells. The fraction of HT cells in a mixture correlated linearly with the ATP/DNA ratio, whereas the ADP/ADP ratio was highly scattered, remaining effectively unchanged. Despite similar limitations in both ADP/ADP and ATP/DNA ratios, in that ATP levels may fluctuate significantly and reversibly with metabolic stress, the results indicated that ATP/DNA was a better measure of islet viability than the ADP/ATP ratio. |
| Databáze: | OpenAIRE |
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