TNF and TNF receptor expression and insulin sensitivity in human omental and subcutaneous adipose tissue--influence of BMI and adipose distribution

Autor: Johannes B. Prins, Manuela Good, Felicity M Newell, Jonathan P. Whitehead, Larisa M. Haupt, L Hutley
Rok vydání: 2006
Předmět:
Adult
Male
medicine.medical_specialty
Endocrinology
Diabetes and Metabolism
medicine.medical_treatment
Glucose uptake
Abdominal Fat
Adipose tissue
030209 endocrinology & metabolism
Type 2 diabetes
030204 cardiovascular system & hematology
Deoxyglucose
Adipocytes/*metabolism
Receptors
Tumor Necrosis Factor
Body Mass Index
03 medical and health sciences
0302 clinical medicine
Insulin resistance
Internal medicine
Diabetes mellitus
Receptors
Internal Medicine
medicine
Adipocytes
Body Fat Distribution
Humans
Insulin
Abdominal Fat/*metabolism
Aged
business.industry
Tumor Necrosis Factor-alpha
Waist-Hip Ratio
Tumor Necrosis Factor/*metabolism
Deoxyglucose/metabolism
Middle Aged
medicine.disease
Endocrinology
medicine.anatomical_structure
Insulin Resistance/*physiology
Tumor necrosis factor alpha
Female
Tumor Necrosis Factor-alpha/*metabolism
Insulin Resistance
Cardiology and Cardiovascular Medicine
business
060103 Cell Development Proliferation and Death
Subcutaneous tissue
Zdroj: Diabetes and Vascular Disease Research
ISSN: 2381-3652
Popis: Tumour necrosis factor (TNF)α is implicated in the relationship between obesity and insulin resistance/type 2 diabetes. In an effort to understand this association better we (i) profiled gene expression patterns of TNF, TNFR1 and TNFR2 and (ii) investigated the effects of TNF on glucose uptake in isolated adipocytes and adipose tissue explants from omental and subcutaneous depots from lean, overweight and obese individuals. TNF expression correlated with expression of TNFR2, but not TNFR1, and TNF and TNFR2 expression increased in obesity. TNFR1 expression was higher in omental than in subcutaneous adipocytes. Expression levels of TNF or either receptor did not differ between adipocytes from individuals with central and peripheral obesity. TNF only suppressed glucose uptake in insulin-stimulated subcutaneous tissue and this suppression was only observed in tissue from lean subjects. These data support a relationship between the TNF system and body mass index (BMI), but not fat distribution, and suggest depot specificity of the TNF effect on glucose uptake. Furthermore, adipose tissue from obese subjects already appears insulin ‘resistant’ and this may be a result of the increased TNF levels.
Databáze: OpenAIRE
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