Dependence of c-fos Expression on Amplitude of High-Frequency Spinal Cord Stimulation in a Rodent Model
| Autor: | Kennon M. Garrett, Robert D. Foreman, Jiande D. Z. Chen, Michael A. Moffitt, Bengt Linderoth, Tianhe Zhang, Jianwen Wendy Gu, Shiying Li, Feng Ye, Jay P. Farber |
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| Rok vydání: | 2019 |
| Předmět: |
Male
Action Potentials Stimulation Spinal cord stimulation c-Fos Biophysical Phenomena Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine medicine Animals Spinal Cord Stimulation biology business.industry General Medicine Spinal cord Sciatic Nerve Low back pain Rats Compound muscle action potential Posterior Horn Cells Anesthesiology and Pain Medicine Amplitude medicine.anatomical_structure Neurology Sensory Thresholds Models Animal biology.protein Neurology (clinical) Sciatic nerve medicine.symptom business Proto-Oncogene Proteins c-fos 030217 neurology & neurosurgery Biomedical engineering |
| Zdroj: | Neuromodulation: Technology at the Neural Interface. 22:272-278 |
| ISSN: | 1094-7159 |
| DOI: | 10.1111/ner.12852 |
| Popis: | OBJECTIVES Clinical high-frequency spinal cord stimulation (hfSCS) (>250 Hz) applied at subperception amplitudes reduces leg and low back pain. This study investigates, via labeling for c-fos-a marker of neural activation, whether 500 Hz hfSCS applied at amplitudes above and below the dorsal column (DC) compound action potential (CAP) threshold excites dorsal horn neurons. MATERIALS AND METHODS DC CAP thresholds in rats were determined by applying single biphasic pulses of SCS to T12 -T13 segments using pulse widths of 40 or 200 μsec via a ball electrode placed over the left DC and increasing amplitude until a short latency CAP was observed on the L5 DC and sciatic nerve. The result of this comparison allowed us to substitute sciatic nerve CAP for DC CAP. SCS at T12 -T13 was applied continuously for two hours using: sham or hfSCS at 500 Hz SCS, 40 μsec pulse width, and 50, 70, 90, or 140% CAP threshold. Spinal cord slices from T11 -L1 were immunolabeled for c-fos, and the number of c-fos-positive cells was quantified. RESULTS 500 Hz hfSCS applied at 90 and 140% CAP threshold produced substantial (≥6 c-fos + neurons on average per slice per segment) c-fos expression in more segments between T11 and L1 than did sham stimulation (p < 0.025, 90% CAP; p < 0.001, 140% CAP, Fisher's Exact Tests) and resulted in more c-fos-positive neurons on average per slice per segment ipsilateral to than contralateral to the SCS electrode at 70, 90, and 140% CAP threshold (p < 0.01, Wilcoxon Signed Rank Tests). CONCLUSIONS The finding of enhanced c-fos expression in the ipsilateral superficial dorsal horn provides evidence for activation/modulation of neuronal circuitry associated with subperception hfSCS. |
| Databáze: | OpenAIRE |
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