Multicolor multiphoton microscopy based on a nanosecond supercontinuum laser source
| Autor: | Rodney P. O'Connor, Philippe Leproux, Vincent Couderc, Laetitia Magnol, Claire Lefort, Véronique Blanquet, Philippe Lévêque, Hideaki Kano, Vincent Tombelaine |
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| Přispěvatelé: | Photonique Fibre et Sources Cohérentes (XLIM-PHOT), XLIM (XLIM), Université de Limoges (UNILIM)-Centre National de la Recherche Scientifique (CNRS)-Université de Limoges (UNILIM)-Centre National de la Recherche Scientifique (CNRS), Université de Limoges (UNILIM)-Centre National de la Recherche Scientifique (CNRS), Unité de Génétique Moléculaire Animale (UMR GMA), Université de Limoges (UNILIM)-Institut National de la Recherche Agronomique (INRA), OSA (XLIM-OSA), 'Defi Imag'In' program from CNRS for the project 'AR-KENCIEL', Region Limousin, Unité de Génétique Moléculaire Animale (UGMA) |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Materials science
nanosecond supercontinuum source General Physics and Astronomy Texas Red multicolor imaging 02 engineering and technology 01 natural sciences General Biochemistry Genetics and Molecular Biology law.invention 010309 optics chemistry.chemical_compound Mice Optics law 0103 physical sciences Animals General Materials Science Muscle Skeletal business.industry Lasers General Engineering Brain General Chemistry Glioma Nanosecond 021001 nanoscience & nanotechnology Laser Supercontinuum Full width at half maximum Microscopy Fluorescence Multiphoton chemistry Femtosecond multiphoton microscopy [SPI.OPTI]Engineering Sciences [physics]/Optics / Photonic Monochromatic color nonlinear imaging 0210 nano-technology business Excitation |
| Zdroj: | Journal of Biophotonics Journal of Biophotonics, Wiley, 2016, 9 (7), pp.709-714. ⟨10.1002/jbio.201500283⟩ Journal of Biophotonics, Wiley, 2016, ⟨10.1002/jbio.201500283⟩ |
| ISSN: | 1864-063X 1864-0648 |
| DOI: | 10.1002/jbio.201500283⟩ |
| Popis: | International audience; Multicolor multiphoton microscopy is experimentally demonstrated for the first time on a spectral bandwidth of excitation of 300 nm (full width half maximum) thanks to the implementation a nanosecond supercontinuum (SC) source compact and simple with a low repetition rate. The interest of such a wide spectral bandwidth, never demonstrated until now, is highlighted in vivo: images of glioma tumor cells stably expressing eGFP grafted on the brain of a mouse and its blood vessels network labelled with Texas Red® are obtained. These two fluorophores have a spectral bandwidth covering the whole 300 nm available. In parallel, a similar image quality is obtained on a sample of mouse muscle in vitro when excited with this nanosecond SC source or with a classical high rate, femtosecond and quasi monochromatic laser. This opens the way for (i) a simple and very complete biological characterization never performed to date with multiphoton processes, (ii) multiple means of contrast in nonlinear imaging allowed by the use of numerous fluorophores and (iii) other multiphoton processes like three-photon ones. |
| Databáze: | OpenAIRE |
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