Local carotid atherosclerotic plaque proteins for the identification of circulating biomarkers in coronary patients

Autor: Francois Rieunier, Daniel Laune, Jeannette Fareh, Dominique Piquer, Bernard Albat, Aurélie Blanc, Eric Malaud, Olivier Meilhac, Agnès Pouzet, Charles Marty-Ané, Franck Molina, Sandra Cobo, Laetitia Rubrecht, Laurence Molina, Saussine M, Emilie Dupaty, Pascale Galea, Nicolas Salvetat, Delphine Merle
Přispěvatelé: BIO-RAD, Université Henri Poincaré - Nancy 1 (UHP), Sys2Diag-Modélisation et Ingénierie des Systèmes Complexes Biologiques pour le Diagnostic (Sys2Diag), Centre National de la Recherche Scientifique (CNRS)-Alcediag, Institut de Recherche en Infectiologie de Montpellier (IRIM), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Service de chirurgie thoracique et cardio-vasculaire, Hôpital Arnaud de Villeneuve-Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier)-Université Montpellier 1 (UM1)-Université de Montpellier (UM), Diabète athérothrombose et thérapies Réunion Océan Indien (DéTROI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de La Réunion (UR), laboratoire de chimie et pharmacologie de molécules d'intérêt biologique
Rok vydání: 2014
Předmět:
Adult
Carotid Artery Diseases
Male
Pathology
medicine.medical_specialty
[SDV]Life Sciences [q-bio]
In silico
medicine.medical_treatment
Hemorrhage
Coronary Artery Disease
030204 cardiovascular system & hematology
Ligands
medicine.disease_cause
03 medical and health sciences
0302 clinical medicine
Platelet degranulation
Peptide Library
Fibrinolysis
medicine
Combinatorial Chemistry Techniques
Humans
Electrophoresis
Gel
Two-Dimensional
Multiplex
ComputingMilieux_MISCELLANEOUS
Peptide ligand
Aged
030304 developmental biology
Aged
80 and over
Inflammation
Endarterectomy
Carotid
0303 health sciences
Rupture
Spontaneous
business.industry
Autophagy
Blood Proteins
Middle Aged
Fibrosis
Vulnerable plaque
Plaque
Atherosclerotic
3. Good health
Circulating biomarkers
Subtraction Technique
Cytokines
Female
Disease Susceptibility
Chemokines
Cardiology and Cardiovascular Medicine
business
Biomarkers
Zdroj: Atherosclerosis
Atherosclerosis, Elsevier, 2014, 233 (2), pp.551-558. ⟨10.1016/j.atherosclerosis.2013.12.019⟩
ISSN: 0021-9150
Popis: To identify circulating biomarkers that originate from atherosclerotic vulnerable plaques and that could predict future cardiovascular events.After a protein enrichment step (combinatorial peptide ligand library approach), we performed a two-dimensional electrophoresis comparative analysis on human carotid plaque protein extracts (fibrotic and hemorrhagic atherosclerotic plaques). In silico analysis of the biological processes was applied on proteomic data. Luminex xMAP assays were used to quantify inflammatory components in carotid plaques. The systemic quantification of proteins originating from vulnerable plaques in blood samples from patients with stable and unstable coronary disease was evaluated.A total of 118 proteins are differentially expressed in fibrotic and hemorrhagic plaques, and allowed the identification of three biological processes related to atherosclerosis (platelet degranulation, vascular autophagy and negative regulation of fibrinolysis). The multiplex assays revealed an increasing expression of VEGF, IL-6, IL-8, IP-10 and RANTES in hemorrhagic as compared to fibrotic plaques (p0.05). Measurement of protein expressions in plasmas from patients with stable and unstable coronary disease identified a combination of biomarkers, including proteins of the smooth muscle cell integrity (Calponin-1), oxidative stress (DJ-1) and inflammation (IL-8), that allows the accurate classification of patients at risk (p=0.0006).Using tissue protein enrichment technology, we validated proteins that are differentially expressed in hemorrhagic plaques as potential circulating biomarkers of coronary patients. Combinations of such circulating biomarkers could be used to stratify coronary patients.
Databáze: OpenAIRE
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