SP1-mediated upregulation of lncRNA LMCD1-AS1 functions a ceRNA for miR-106b-5p to facilitate osteosarcoma progression
| Autor: | Yalong Zhu, Jianhua Zhou, Dejian Li, Baoqing Yu, Jia-wen He |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
musculoskeletal diseases
0301 basic medicine Male Epithelial-Mesenchymal Transition Sp1 Transcription Factor Biophysics Bone Neoplasms Biology Biochemistry Metastasis 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation Cell Line Tumor medicine Gene silencing Humans neoplasms Molecular Biology Cell Proliferation Osteosarcoma Competing endogenous RNA Promoter Cell Biology medicine.disease Antisense RNA Up-Regulation Gene Expression Regulation Neoplastic MicroRNAs 030104 developmental biology 030220 oncology & carcinogenesis Cancer research Disease Progression Biomarker (medicine) Female RNA Long Noncoding |
| Zdroj: | Biochemical and biophysical research communications. 526(3) |
| ISSN: | 1090-2104 |
| Popis: | Growing studies have indicated the involvements of long noncoding RNAs (lncRNAs) in the initiation and progression of various tumors. We aimed to investigated the role of lncRNA LMCD1 antisense RNA 1 (LMCD1-AS1) in osteosarcoma development. We found that LMCD1-AS1 and SP1 were highly expressed in osteosarcoma tissues and cell lines. High levels of LMCD1-AS1 were correlated with positively metastasis and poor clinical prognosis. Moreover, we showed that SP1 can bind to the promoter region of LMCD1-AS1, resulting in its overexpression in osteosarcoma. Functionally, silencing of LMCD1-AS1 suppressed the proliferation, migration, invasion and EMT progress of osteosarcoma cells. Mechanistic studies revealed that LMCD1-AS1 was a sponge of miR-106b-5p activity. LMCD1-AS1 modulated survival of osteosarcoma via targeting miR-106b-5p. Overall, we firstly indicated that LMCD1-AS1 overexpression contributes to osteosarcoma development and poor clinical outcome, suggesting that LMCD1-AS1 may be a novel diagnostic and prognostic biomarker for osteosarcoma and a target for osteosarcoma therapy. |
| Databáze: | OpenAIRE |
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